Registration Dossier
Registration Dossier
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EC number: 601-818-6 | CAS number: 122009-11-4
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study in compliance with GLP-criteria.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 4-(diethoxyphosphinyl)-2-methyl-2-butenoic acid ethyl ester
- EC Number:
- 601-818-6
- Cas Number:
- 122009-11-4
- Molecular formula:
- C11 H21 O5 P
- IUPAC Name:
- 4-(diethoxyphosphinyl)-2-methyl-2-butenoic acid ethyl ester
- Details on test material:
- - Name of test material: C5-Ester-Phosphonat
- Aggregation state: liquid
- Homogeneity: homogenous
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: 30 mg/L from a laboratory wastewater treatment plant, which was mixed (80:20) with municipal sewage and synthetic wastewater.
- Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 20 mg/L
- Based on:
- DOC
- Initial conc.:
- 38.5 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- DOC removal
- Details on study design:
- Number of replicates:
- Test substance: 2
- Blank control: 1
- Reference control: 1
- Abiotic control: 1
- Inhibition control: 1
Test solution preparation:
- Inorganic medium: 13 mL
- Destilled water: 944 mL
- Test substance solution: 39 mL
- Total liquid volume: 1000 mL
- Inoculum: 5 mL
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (DOC removal)
- Value:
- 39
- Sampling time:
- 28 d
- Details on results:
- Kinetic of test substance (in %):
< 0 after 10 day(s)
= 20 after 14 day(s)
= 26 after 24 day(s)
= 35 after 27 day(s)
= 39 after 28 day(s)
BOD5 / COD results
- Results with reference substance:
- 89% degradation after 1 day
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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