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EC number: 218-414-7 | CAS number: 2146-71-6
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1990-11-07 until 1991-07-31
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: OECD Guideline study under GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
- Report date:
- 1991
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Vinyl laurate
- EC Number:
- 218-414-7
- EC Name:
- Vinyl laurate
- Cas Number:
- 2146-71-6
- Molecular formula:
- C14H26O2
- IUPAC Name:
- ethenyl dodecanoate
- Details on test material:
- - Name of test material (as cited in study report): Vinyllaurat
- Physical state: colourless liquid
- Impurities (identity and concentrations):not given in report
- Composition of test material, percentage of components: as stated in substance data
- Lot/batch No.: 959837
- Stability under test conditions: stable
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
not applicable
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source: domestic waste water treatment plant (Kläranlage Karlsruhe/Neureut 1991-02-20)
- Method of cultivation: not given in report - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 1 mg/L
- Based on:
- test mat.
- Initial conc.:
- 2 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: in accordance with Guideline
- pH adjusted: no
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus:2 litre glass bottles
- Number of culture flasks/concentration: 2 bottles (test substance + inoculum), 2 bottles (inoculum),2 bottles (reference compound sodium acetate + inoculum), 2 bottles (test substance + reference compound sodium acetate + inoculum)
- Test substance: test substance was directly mixed with test medium
SAMPLING
- Sampling frequency: measurement was performed on exposure days 0, 5, 7, 16 and 28
CONTROL AND BLANK SYSTEM
- Inoculum blank: inoculum+ test medium
- Procedure control: soium acetate + inoculum/ test medium
- Toxicity control: test substance + sodium acetate + inoculum
Reference substance
- Reference substance:
- acetic acid, sodium salt
Results and discussion
- Preliminary study:
- not applicable
- Test performance:
- according to guideline
% Degradationopen allclose all
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 0 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 74
- Sampling time:
- 5 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 87
- Sampling time:
- 16 d
- Parameter:
- % degradation (O2 consumption)
- Value:
- 98
- Sampling time:
- 28 d
- Details on results:
- The reference compound sodium acetate was biodegraded by 87 % by exposure day 16 and reached an biodegradation rate of 84% by the end of the study, thus confirming suitability of the activated sludge.
BOD5 / COD results
- Results with reference substance:
- The reference compound sodium acetate was biodegraded by 87 % by exposure day 16 and reached an biodegradation rate of 84% by the end of the study, thus confirming suitability of the activated sludge.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test item Vinyl laurate is readily biodegradable under the conditions used.
- Executive summary:
Vinyl laurate was investigated for its ready biodegradability in the "Closed Bottle Test" (OECD 301 D) when exposed to aerobic activated sludge from a domestic waste water treatment plant over a period of 28 days. Under the test conditions Vinyl laurate was found to be readily biodegradable over the 28-day exposure period. The reference substance sodium acetate reached the level for ready biodegradability, thus confirming suitability of the activated sludge. In the the inhibition test no inhibition of the bacterial growth was noted.
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