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EC number: 600-033-6
CAS number: 1001416-18-7
The toxicity of the substance towards waste
water bacteria was assessed in an assay according to OECD Guideline 209
(Activated Sludge, Respiration Inhibition Test, 1984). Since the
substance was hardly soluble in water test concentrations were prepared
separately in Milli-RO water. Two 1 litre test bottles were filled with
250 ml of test substance mixtures with initial loading rates of 200
mg/L. These mixtures were stirred in dark glass closed bottles for 24
hours. Subsequently, 16 ml synthetic sewage feed, 200 ml activated
sludge and Milli-RO water up to 500 ml were added resulting in a loading
rate of 100 mg/L. This loading rate was tested in duplicate. Optimal
contact between the test substance and test medium was ensured applying
continuous aeration and stirring. Thereafter oxygen consumption was
measured and recorded for approximately 10 minutes.
No significant inhibition of respiration
rate of the sludge was recorded at a loading rate of 100 mg/L. The
duplicate measurement confirmed the results of the first measurement.
Therefore no further testing was necessary.
The EC50 of the substance exceeded a loading
rate of 100 mg/L (based on nominal concentrations).
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