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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study, no deviations

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2009
Report Date:
2009

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
- Concentrations: 100 mg/L (loading rate)
- Sampling method: 2 ml sample was aken from the test solution
- Sample storage conditions before analysis: Samples were stored in a freezer befor analysis
- Frequency of sampling: at t = 0 h, t = 24 h, t 0 96 h

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A test solution with a loading rate of 100 mg/L was prepared for the limit test. The test solution was stirred for 1 hour before a stbilisation period of 1 hour. The resulting solution was slightly hazy (pre-incubation of the test vessels) or clear and colourless (limit study) with a floating layer. The apparent water soluble fraction of the solution was then siphoned off over glass wool befor use as a test concentration.
Note that the test vessels were pre-incubated with the test solution one day prior to the start of the experiments in order to minimise possible adsorption to glass ware.

- Controls: Test medium without test substance or other additives

Test organisms

Test organisms (species):
Cyprinus carpio
Details on test organisms:
TEST ORGANISM
- Common name: Carp
- Source: Zodiac, proefacc, "De Haar Vissen", Wageningen University and Research Centre, The Netherlands
- Age at study initiation: No data
- Length at study initiation: 2.5 +/- 0.1 cm
- Weight at study initiation: 0.46 +/- 0.04 g
- Method of breeding: F1 from a single parent-pair bred in UV-treated water
- Feeding during test
- Food type: Pelleted fish food (Cyprico crumble Excellent (300-500 µm), Coppens International bv, Helmond, The Netherlands)
- Amount: No data
- Frequency: Daily

ACCLIMATION
- Acclimation period: At least 12 after delivery
- Acclimation conditions (same as test or not): No data
- Type and amount of food: No data
- Feeding frequency: No data
- Health during acclimation (any mortality observed): No data

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Post exposure observation period:
n.a

Test conditions

Hardness:
180 mg CaCO3/L
Test temperature:
21.6 °C at day 0 - 22.7 °C at day 4
pH:
7.7 +/- 0.3
Dissolved oxygen:
9.2 mg/L at day 0 - 6.0 mg/L at day 4
Salinity:
No data
Nominal and measured concentrations:
Nominal concentration: Load: 100 mg/L
Measured concentrations:
t = 0: 1.39 mg/L
t = 24 h: 1.28 mg/L
t = 96 h: 2.11 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: All glass vessels
- Type (delete if not applicable): open / closed
- Material, size, fill volume: Glass, 10 litres; fill volume: 9 litres
- Aeration: The test media were not aerated during the test.
- Renewal rate of test solution (frequency/flow rate): The test system was not renewed
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): n.a.
- Biomass loading rate: not determined

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water, purified by reverse osmosis
- Total organic carbon: not determined
- Particulate matter:not determined
- Metals: not determined
- Pesticides: not determined
- Chlorine: not determined
- Alkalinity: not determined
- Ca/mg ratio: not determined
- Conductivity: not determined
- Culture medium different from test medium: No data
- Intervals of water quality measurement: No data

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16 hours photoperiod daily
- Light intensity: Low light intensity

EFFECT PARAMETERS MEASURED: Mortality

TEST CONCENTRATIONS
- Spacing factor for test concentrations: N.a. as test was a limit test

- Range finding study: No
- Test concentrations:
Nominal concentration: Load: 100 mg/L
Measured concentrations:
t = 0: 1.39 mg/L
t = 24 h: 1.28 mg/L
t = 96 h: 2.11 mg/L
Reference substance (positive control):
yes
Remarks:
Pentachlorophenol

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 1.3 - 2.1 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Behavioural abnormalities: None observed
- Observations on body length and weight: Not determined
- Other biological observations: None
- Mortality of control: None
- Other adverse effects control: None
- Abnormal responses: None
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: None
- Effect concentrations exceeding solubility of substance in test medium: N.a.
Results with reference substance (positive control):
- Results with reference substance valid? Yes
- Initial number of fish: 5
- Mortality:
0% at a conc. of 0.10 mg/L after 96 h
40 % at a conc. of 0.22 mg/L after 24 h
60 % at a conc. of 0.22 mg/L after 48 h
100 % at a conc. of 0.46 mg/L after 24 h
- LC50: 0.2 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Under the conditions of the test the substance induced no visible or lethal effects in carp up to the limit of solubility in test medium.
The 96 h LC 50 exceeded the water soluble fraction of the test substance obtained after glass-wool filtration of a loading rate prepared at 100 mg/l, with an actual concentration ranging between 1.3 and 2.1 mg/L.
Executive summary:

The toxicity of the substance to carp was assessed in a study according to OECD Guideline 203 (Fish, Acute Toxicity Test, 1992). A limit test was performed consisting of a blank control and a maximum soluble fraction of the substance in test medium. The total test period was 96 hours and samples for analytical confirmation of actual exposure concentrations were taken at the start, after 24 hours and at the end of the test period.

Analyses of samples taken from the glass-wool filtered solution of 100 mg/L showed that the measured concentration varied between 1.3 and 2.1 mg/L during the test period. These concentrations were at or slightly above the expected concentrations based on the solubility in pure water.

Under the conditions of the test the substance induced no visible or lethal effects in carp up to the limit of solubility in test medium. The 96 h LC50 exceeded the water soluble fraction of the test substance obtained after glass-wool filtration of a loading rate prepared at 100 mg/l, with an actual concentration ranging between 1.3 and 2.1 mg/L.