Registration Dossier

Administrative data

Endpoint:
partition coefficient
Type of information:
experimental study
Adequacy of study:
key study
Study period:
29 April - 25 July, 2008
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: The study has been performed according to OECD and/or EC guidelines and according to GLP principles.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2008
Report Date:
2008

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 117 (Partition Coefficient (n-octanol / water), HPLC Method)
Deviations:
no
Qualifier:
according to
Guideline:
EU Method A.8 (Partition Coefficient)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Type of method:
HPLC method
Partition coefficient type:
octanol-water

Test material

Reference
Name:
Unnamed
Type:
Constituent

Study design

Analytical method:
high-performance liquid chromatography

Results and discussion

Partition coefficient
Type:
log Pow
Partition coefficient:
> 6.5
Temp.:
22
pH:
ca. 7
Details on results:
In the chromatogram of the test substance solution no test substance peak was observed during isocratic elution with 75/25 (v/v) methanol/water. Under the same analytical conditions, the retention time of 2,4-DDT (log Pow = 6.5) was 26.0 minutes. Yet, more than ten peaks related to the test substance were observed between 34.5 and 41.4 minutes, which is during the column rinse, i.e. under gradient conditions. Hence, it was concluded that the log Pow of the components of the test substance was > 6.5 (Pow > 3.2 x 10^6).

Any other information on results incl. tables

The Pow of the test substance was calculated to be 3.7 x 106 (log Pow = 6.57) using the Rekker calculation method.

Applicant's summary and conclusion

Conclusions:
The results of the Rekker calculation method and the HPLC method differ more than 0.3 log units. Since the HPLC method is a more accurate method, the results of this method are reported. The Pow and log Pow values of the components of LE 5288 are >3.2 x 10^6 and >6.5, respectively.
Executive summary:

The octanol/water partition coefficient of the substance was determined according to OECD Guideline 117. The results of the Rekker calculation method and the HPLC method differ more than 0.3 log units. Since the HPLC method is a more accurate method, the results of this method are reported. The Pow and log Pow values of the components of LE 5288 are >3.2 x 10^6 and >6.5, respectively.