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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Method in accordance with OECD Guideline, GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report Date:
1992

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
purity: > 98.5 %
Batch TD 90-133

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
other: activated sewage sludge bacteria
Details on inoculum:
Aeration stage of the HRC Limited sewage treatment plant treating predominantly domestic sewage
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
3 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
other: Percent biodegradation determined by comparing oxygen depletion value with the corresponding ThOD
Details on study design:
Sampling at 0, 5, 15 and 28 days
20°C
Reference substance
Reference substance:
benzoic acid, sodium salt

Results and discussion

% Degradation
Parameter:
% degradation (O2 consumption)
Value:
86
Sampling time:
28 d
Remarks on result:
other: Oxygen depletion = 4.325 mg O2/L; ThOD = 5.01 mg O2/L
Details on results:
Oxygen depletion in the inoculated and non-inoculated control series were within prescribed limits.
The substance passed the "time window" criterion (60% degradation within 10 days of attaining the 10% level)

BOD5 / COD results

Results with reference substance:
87% degradation within 28 days

Any other information on results incl. tables

Mean oxygen depletion and percentage biodegradation values

Culture medium

Day 5

Day 15

Day 28

(a) Dilution water without inoculum

    02depletion (mgO2/I)

 

0.175

 

0.275

 

0.250

(b) Dilution water with inoculum

    02depletion (mgO2/I)

 

0.100

 

0.300

 

0.375

(c) Test substance (3 mg/l)

    02depletion (mgO2/I)

    % degradation

 

3.050

61

 

3.000

60

 

4.325

86

(d) . Standard substance (3 mg/l)

    02depletion (mgO2/I)

    % degradation

 

3.275

65

 

3.450

69

 

4.375

87

t1/2: 10.3 days

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
In a valid guideline study, the substance was considered readily biodegradable (86% degradation within 28 days, 10 day window passed).
Executive summary:

In a valid guideline study acc. OECD 301 D (Readily Biodegradability: Closed Bottlte Test) methacrylic acid attained 86 % biodegradation within 28 days. The"pass" level of 60 % was reached within 10 days of exceeding 10 % level. Therefore methacrylic acid can be termed as readily biodegradable.

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