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EC number: 212-084-8 | CAS number: 760-93-0
Methacrylic anhydride was sensitising to skin in an OECD 429 Local Lymphnode assay.
Calculation and Results of Individual Data
Number of lymph nodes
DPM per lymph node
BG=Background (1 ml 5% trichloroacetic acid) in duplicate
a)=The mean value was taken from the figures BG land BG11
b)=Since the Iymph nodes of the an im als of a dose group were pooled, DPM/node was determined by dividing the measured value by the number of Iymph nodes
No deaths occurred during the study period.
The animals did not show any clinical signs of toxicity after the first and second application. After the third application the mid (50 %) and the high dose (100 %) induced redness of the ear skin of all four animals of the group.
The body weight of the animals, recorded prior to the first application and prior to treatment with ³HTdR, was within the range commonly recorded for animals of this strain and age.
In a dermal sensitization study with Methacrylic anhydride (95.12%) dissolved in acetone : olive oil (4 +1) as a vehicle, 16 (4per dose group) 7 -8 week old female CBA/CaOlaHsd mice were tested using the method of OECD 429 (Local Lymphnode Assay). The validation-/positive control experiment was performed with alpha-Hexyl cinnamic aldehyde.
The animals did not show any clinical signs of taxi city after the first and second application. After the third application the mid (50 %) and the high dose (100 %) induced redness of the of the ear skin of all four animals of the group. No cases of mortality were observed.
In this study Stimulation Indices (S.I.) of 12.06, 13.69 and 14.57 were determined with the test item at concentrations of 25, 50, and 100% in acetone : olive oil (4 +1), respectively. The EC3 value could not be calculated, since all SI´s were above 3. The effect was due to the sensitising potential of the test item even though reddening of the ears occurred at the mid and high dose. Even at the lowest tested concentration where no redness of the ears was observed, the S.I. was well above the threshold for sensitisers.
For the evaluation of the effect strength however, also the acidic properties of MAA as hydrolysis product and primary metabolite of MAAH have to considered.
CLP EU GHS (Regulation (EC) No 1272/2008) classification: sensitizing category 1A
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In a mouse local lymphnode assay acc. OECD 429 methacrylic anhydride was found to be sensitising to skin Stimulation Indices (S.I.) of 12.06, 13.69, and 14.57 were determined with the test item at concentrations of 25, 50, and 100 % in acetone: olive oil (4 + 1), respectively). For the evaluation of the effect strength, the acidic properties of MAA as hydrolysis product and primary metabolite of MAAH have to considered. Justification for selection of skin sensitisation endpoint: One GLP Guideline study available with Klimisch score 1.
Methacrylic anhydride fulfils the criteria for skin sensitising category 1 according to CLP (1272/2008/EC) and UN GHS.
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