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Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Method sufficiently described.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1982

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 402 (Acute Dermal Toxicity)
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Purity: 99.8% MMA

Test animals

Species:
rabbit
Strain:
New Zealand White
Sex:
male
Details on test animals and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 2.55 - 2.8 kg

Administration / exposure

Type of coverage:
occlusive
Vehicle:
unchanged (no vehicle)
Details on dermal exposure:
REMOVAL OF TEST SUBSTANCE
After the 24-h exposure, the cuffs were removed and the application sites were gently wiped with paper towels to remove the test substance. Despite this procedure, some of the test substance remained on the skin and fur staining them yellow. Subsequently animals were observed preening areas of treated skin.
Duration of exposure:
24 hrs
Doses:
0.2, 2.0 and 5.0 g/kg
No. of animals per sex per dose:
two males per dose
Control animals:
no
Details on study design:
Animals were observed 14 days post-dosing

Results and discussion

Effect levels
Sex:
male
Dose descriptor:
LD50
Effect level:
> 5 000 mg/kg bw
Mortality:
There were no deaths observed
Clinical signs:
There were no clincial signs observed
Body weight:
not reported in full detail
Gross pathology:
There were no gross necropsy changes observed
Other findings:
There was well defined to severe erythema with blanching, and moderate to severe edema with pocketing were observed at 24 hr at one or more dose levels. Skin irritation did not disappear at 14 d at 2 or 5 g/kg but did disappear at day 3 at 0.2 g/kg. Eschar was observed at day 2 at 2 and 5 g/kg but some eschar was observed to be sloughing off with new hair growth on the underlying skin at day at both dose levels. Dessication was observed after day 4 at all dose levels.

Applicant's summary and conclusion