Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 428-650-4 | CAS number: 153719-23-4
Table: Effects of the test substance on emergence and development of Chironomus riparius after 30 days exposure for Scenario A
Nominal concentrations (µg a.s./L)
Mean number emerged
Mean emergence rate
Mean gender rate
Mean development rate/vessel
Day to first hatch
Table:Effects of the test substance on emergence and development of Chironomus riparius after 30 days exposure for Scenario B
Nominal concentrations (µg a.s./kg dry sediment)
Table: Summary of the effects of the test substance on growth of Chironomus riparius after 7 days exposure for Scenario A
Concentration (µg a.s./L)
Average dry weight of larvae (mg)
No. larvae found
Table: Summary of the effects of the test substance on growth of Chironomus riparius after 7 days exposure for Scenario B
Concentration (µg a.s./kg dry sediment)
0 (solvent control)
The toxicity to sediment-dwelling organisms was determined in a GLP-compliant study according to OECD Proposal for toxicity test with Chironomidae. In this study, harlequin fly larvae (60 per treatment; Chironomus rapirius) were exposed through a water (Scenario A) and sediment (Scenario B) system to nominal test substance concentration of 0 (control), 1.25, 2.5, 5, 10, 20 and 40 µg a.s./L and 0 (control), 0 (vehicle control) 12.5, 25, 50, 100, 200 and 400 µg a.s./kg dry sediment, respectively for 30 days. For scenario A, the substance was dissolved in the medium. For scenario B, the test substance was spiked in artificial soil that contained 68% industrial sand, 20% koalin clay and 10% sphagnum peat moss. Visual assessments of behaviour, mortalities and emergence were made on most days for both scenarios. Emerged adults were removed from the test vessels when seen, recorded and sexed before being discarded. The emergence and development rates, and the gender ratio were calculated from the total numbers of emerged male and female adults, and from the time to emergence. On day 7 a sacrificial replicate at each treatment and control, set up at the same time as the three test replicates, was sieved to remove the larvae. After drying at 60 °C the larvae were weighed. For the water and sediment tests, no signs of toxicity were observed up to 10.0 µg/L and 100 µ/kg dry sediment, repsectively, throughout the test. The the two highest concentrations in both scenarios were used as sacrificial replicates and were therefore not used for determination of toxic effects of the test substance. Based on these findings, the 30-d NOEC values for emergence rate were determined to be > 10 µg a.s./L and 100 µg a.s./kg dry sediment, for scenario A and B respectively. The derived 30-d EC50 values for emergence rate were determined to be 11.4 a.s./L and 114 µg a.s./kg dry sediment, for scenario A and B respectively.
All available data were assessed and the studies representing the worst-case effects were included as key or weight-of-evidence studies. Other studies are included as supporting information. The key studies are considered to be worst-case and were selected for the CSA.
30-d NOEC = 100 µg a.s./kg dry sediment, Chironomus riparius, emergence rate, OECD Proposal for toxicity test with Chironomidae (1997), Grade 1998
Table: Overview of available data on the toxicity to sediment-dwelling organisms
Guideline / GLP
OECD Proposal for toxicity test with Chironomidae (1997)
Several deviations from current guidelines were observed. Most validation criteria were met. The 30-d NOEC of > 10 µg/L is used in the aquatic hazard evaluation as the most sensitive aquatic invertebrate value.
114 µg/kg dry sediment
100 µg/kg dry sediment
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Close Do not show this message again