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Adsorption / desorption

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Reference
Endpoint:
adsorption / desorption: screening
Type of information:
experimental study
Adequacy of study:
key study
Study period:
September 15 - January 2016
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))
GLP compliance:
yes (incl. certificate)
Type of method:
HPLC estimation method
Media:
other: LiChrospher CN 100A 250 × 4.6 mm, 5µm
Specific details on test material used for the study:
- Batch number: NJC1506009
- Appearance: White crystals
- Manufacturing date: 11 June 2015
- Expiry date: 10 December 2016
- Storage conditions: Controlled room temperature (15-25°C, below 70 RH%), under inert gas, protected from humidity
- Safety precautions: Routine safety precautions (lab coat, gloves, safety glasses, face mask) for unknown materials were applied to assure personnel health and safety.
Radiolabelling:
no
Test temperature:
Room temperature
Details on study design: HPLC method:
HPLC-SYSTEM & CONDITIONS:
-Merck-Hitachi LaChrom HPLC system:
D-7000 Interface
L-7100 HPLC pump
L-7200 Autosampler
L-7400 UV Detector
L-7612 Degasser
655A-52 Column thermostat
- Balance: BP221S Sartorius
- Ultrasonic bath: Elmasonic S300H, ELMA
- Water purification system: MILLIPORE, DIRECT Q8 UV
- Detector: UV at 220 and 254 nm
- Column: LiChrospher CN 100A 250 × 4.6 mm, 5µm
- Mobile Phase: Acetonitrile: ultrapure water = 6 : 4
- Flow: 1 ml/min.
- Injection volume: 10 ul
- Temperature: 25 °C

REFERENCE SUBSTANCES:
Six chemicals for which log Koc has been reported were used to calibrate the elution time in units of log Koc. Dead time, t0 was determined by the injection of sodium nitrate solution.
Chemical name CAS Number log Koc Purity % Batch No.
Sodium nitrate 7631-99-4 t0 100.8 PP/2011/09731
Acetanilide 103-84-4 1.25 99.9 S6921344
Methyl benzoate 93-58-3 1.80 99.7 S6046630
Triazophos 24017-47-8 2.55 98.9 SZBD102XY
Naphthalene 91-20-3 2.75 99.9 S6258846
Fenthion 55-38-9 3.31 96.2 SZBB010XV
alpha-Endosulfan 959-98-8 4.09 99.6 SZB8289XV

PREPARATION OF TEST SOLUTIONS:
Reference substances were dissolved in acetonitrile. Concentration of these stock solutions was about 1 mg/ml. These stock solutions were diluted with eluent resulting in about 10 µg/ml solutions. In case of Sodim Nitrate the 1 mg/ml solution was prepared. Test item was dissolved in acetonitrile. The concentration of this solution was about 1 mg/ml. This stock solution was diluted with eluent resulting in 10 mg/ml solution.

DETERMINATION OF RETENTION TIMES:
Three series of the above reference solutions and the test item solution were measured alternately, with two replicate injections. For the determination of t0 (t0 = retention time of the unretarded component) sodium nitrate solution was injected. The retention times were determined.
Key result
Sample No.:
#1
Type:
log Koc
Value:
< 0.155 dimensionless
Temp.:
25 °C
Matrix:
HPLC-column
Key result
Sample No.:
#1
Type:
Koc
Value:
< 1 dimensionless
Temp.:
25 °C
Matrix:
HPLC-column
Details on results (HPLC method):
The test item elutes with retention time of 1.41 minutes, before the first reference item having log Koc and Koc, so the calibration cannot be apply. Therefore the log Koc and Koc are lower than 0.155 and 1.00 (the logKoc and Koc of the first reference item).
Validity criteria fulfilled:
yes
Conclusions:
The test item elutes with retention time of 1.41 minutes, before the first reference item having log Koc and Koc, so the calibration cannot be apply. Therefore the log Koc and Koc are lower than 0.155 and 1.00 (the logKoc and Koc of the first reference item).
Executive summary:

The test item was investigated for its adsorption/desorption behaviour in a screening test, in accordance with EC-method C.19 (HPLC-method). The test item elutes with retention time of 1.41 minutes, before the first reference item having log Koc and Koc, so the calibration cannot be apply. Therefore the log Koc and Koc are lower than 0.155 and 1.00 (the logKoc and Koc of the first reference item).

Description of key information

The test item was investigated for its adsorption/desorption behaviour in a screening test, in accordance with EC-method C.19 (HPLC-method). The test item elutes with retention time of 1.41 minutes, before the first reference item having log Koc and Koc, so the calibration cannot be apply. Therefore the log Koc and Koc are lower than 0.155 and 1.00 (the logKoc and Koc of the first reference item).

Key value for chemical safety assessment

Koc at 20 °C:
1

Additional information

Source: GLP-report

[LogKoc: 0.155]