Phthalide

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: guideline study (with GLP and analytics)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1998

Report date:

1998

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Phthalide

EC Number:

201-744-0

EC Name:

Phthalide

Cas Number:

87-41-2

Molecular formula:

C8H6O2

IUPAC Name:

1(3H)-Isobenzofuranone

Details on test material:

- Name of test material (as cited in study report): Phthalide
- Analytical purity: about 100%

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

-Method: The test solution was prepared by adding the test item to the test media using an ultra-turrax without any further pre-treatment. Then, the fish were placed into the test aquaria.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish (Danio rerio)
- Source: Hoechst Marion Russel Germany, GmbH
- Age at study initiation: about 4 months
- Length at study initiation (length definition, mean, range): 2.8 cm (2.5 - 3.2 cm)
- Weight at study initiation (mean and range): 0.24 g (0.1-0.4 g)
- Feeding during test: Withdrawal of food 1 day before and during exposure

ACCLIMATION
- Acclimation period: about 10 days
- Health during acclimation (any mortality observed): mortality of 1% during last 14 days

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

22 °C

pH:

7.9-8.4

Dissolved oxygen:

4.5-9.1 mg/L

Nominal and measured concentrations:

nominal (mg/L): 0, 46.4, 100, 315, 464, 1000;
measured, after 1 hour (mg/L): below limit of detection, 42.5, 85.4, 205.0, 381.9, 773.3;
measured, after 96 hours (mg/L): below limit of detection, 39.2, 82.2, 190.3, 423.6, not determined.

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: All glass aquaria (30 x 22 x 24 cm), Volume of water: 10 L
- No aeration
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.24 g fish/L test water


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Municipal water of the city of Frankenthal, not chlorinated and passed through a charcoal filter, aerated.
- pH about 8.0-8.6
- Ca/Mg ratio: (90 mg/L)/(10 mg/L)


OTHER TEST CONDITIONS
- Photoperiod: 16 : 8 hours day-night regime


EFFECT PARAMETERS MEASURED:
observations: mortality and symptoms after 1, 4, 24, 48, 72, and 96 h
measurements: pH, oxygen and temperature after 1, 24, 48, 72 and 96 h

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

82 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

behaviour

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 190 - < 424 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: Symptoms of intoxication: Narcotic-like state, apathy (oserved at concentrations of 190 mg/L and above).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes