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Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Short term toxicity to fish:

Aim of this study was to determine the nature and effect of test chemical when comes in contact withOryzias latipes. Test conducted according to the OECD Guideline 203 (Fish, Acute Toxicity Test).Test performed under the semi-static condition for the total incubation period of 96 hrs. 0.1 mL/L vehicle was used for the preparation of test solution. 5 L test solution were used in the study. Total amount of the test solution was renewed every 24 hours. Oryzias latipes (Japanese rice fish) is a freshwater fish. 10 fishes per concentration were tested with the tested solution as well as with control.Based on the mortality of fish Oryzias latipes, because of the contact with chemical , test chemical lethal concentration (LC50) was determine to be 1.7 mg/l after the exposure period of 96 hrs. Based on the LC50, chemical was consider as toxic and classified as aquatic acute 2 as per the CLP classification criteria.

Short term toxicity to aq. invertebrates:

Study was assess to evaluate the nature of test chemical chemical on the growth of aquatic invertebrates daphnia magna for the exposure of 96 hrs. Study was conducted according to the OECD Guideline 202 (Daphnia sp., Acute Immobilisation Test and Reproduction Test).

Test performed under the semi-static condition for the total incubation period of 48 hrs. 100 mL/L vehicle were used for the preparation of 100 ml of test solution. Total amount of test solution was renewed after 24-hour exposure. Chemical tested at various concentrations 0, 0.10, 0.22, 0.46, 1.0, 2.2 mg/L (nominal concentrations) and also on vehicle (100 microl/l). 5 daphnia magna per vessel added and the test repeated 4 times. Room light, 16 hours light / 8 hours dark provided to the test organism.After the exposure of test chemical for 48 hrs effect were observed on the daphnia magna. After 24 hrs, the EC50 was 0.92 mg/l and after 48 hrs the NOEC was determine 0.22 mg/l. The EC50 was determine to be 0.61 mg/l with the 95 % CL of 0.49 - 0.75 mg/L. Based on the EC50, chemical was consider as toxic and classified as aquatic acute 1 as per the CLP classification criteria.

Long term toxicity to invertebrates:

Study was assess to evaluate the long term effect of test chemical on the growth and reproduction Inhibition of aquatic invertebrates daphnia magna for the exposure of 21 days. Test conducted according to the OECD Guideline 211 (Daphnia magna Reproduction Test). 100 microL/L [mixed solution of Dimethylformamide / Hydrogenated castor oil (HCO-40) = 3/1 (w/w)] used as a vehicle. Test conducted under the Semi-static method at different concentrations control, vehicle control, 0.022, 0.046, 0.10, 0.22, 0.46 mg/L (geometric ratio: 2.2). 80 ml/vessel test solution added with the total amount of the test solution was renewed every 24 hours. 1 test organism added in each vessel and performed in the 10 replicates. After the exposure of test chemical with the aquatic invertebrates daphnia magna for 21 days, effect concentration EC50 was determine to be 0.46 mg/l and the NOEC was 0.22 mg/l. Based on the EC50 chemical was consider as toxic and can be consider to be classified as aquatic chronic 3 category as per the CLP classification criteria.The test substance is redialy biodegradable in aquatic environment and hence , based on that we can consider that the test substance is not classified as per CLP criteria.

Toxicity to aq. algae and cyanobacteria:

Study was assess to evaluate the nature and effect of test chemical on the growth of green algae for the exposure period of 72 hrs. Study was conducted under the static system. After the exposure effect was determine. Based on the growth rate inhibition of green algae due to the exposure of test chemical , the EC50 and NOEC was 0.012 mg/l and 0.0029 mg/l. But on the basis of Biomass and area under the growth curve, the EC50 and NOEC was determine to be 0.09 mg/l and 0.017 mg/l. Based on the EC50, test chemical was concluded as toxic and can be consider to be classified as aquatic acute 1 as per the CLP classification criteria.

Toxicity to microorganisms:

Aim of this study was to determine the toxic nature of test chemical on the growth of microorganism Tetrahymena pyriformis for the total exposure period of 48 hrs. Chemical purchased from Aldrich Chemical Co., Milwaukee, Wisconsin having 95 % purity. Test performed in 3 replicates and each replicate was a five-step graded concentration series using freshly prepared stock solutions. Only replicate with control cultures in the late log-growth-phase (absorbance of 0.6 to 0.9) were used in these studies. Based on the growth rate inhibition of test organisms Tetrahymena pyriformis due to the exposure of test chemical for 48 hrs, the IGC50 was determine to be 586 mg/l.

Additional information

Summarized result for the determination of nature of test chemical on the growth and other biological and physical activity of fishes, aquatic invertebrates, algae and cyanobacteria and microorganisms when chemical comes in contact with test organisms, by considering the data for test chemicals are as follows: 

 

Short term toxicity to fish:

Based on the various experimental and predicted data for the target chemical and experimental data for structurally and functionally similar read across chemicals study have been reviewed to determine the toxic nature of target chemical 1-Tridecanol (112-70-9) on the fishes. The studies are as mentioned below:

Aim of this study was to determine the nature and effect of test chemical when comes in contact withOryzias latipes. Test conducted according to the OECD Guideline 203 (Fish, Acute Toxicity Test).Test performed under the semi-static condition for the total incubation period of 96 hrs. 0.1 mL/L vehicle was used for the preparation of test solution. 5 L test solution were used in the study. Total amount of the test solution was renewed every 24 hours. Oryzias latipes (Japanese rice fish) is a freshwater fish. 10 fishes per concentration were tested with the tested solution as well as with control.Based on the mortality of fish Oryzias latipes, because of the contact with chemical , test chemical lethal concentration (LC50) was determine to be 1.7 mg/l after the exposure period of 96 hrs. Based on the LC50, chemical was consider as toxic and classified as aquatic acute 2 as per the CLP classification criteria.

 

First experimental study was supported by the second supporting study from peer reviewed journal.

Aim of this study was to determine the nature and effect of test chemical when comes in contact withpimephales promelas (Fathead minnow). Chemical was less soluble thus first extracted into hexane or hexane/ether (75: 25) and analyzed on an appropriate GC column utilizing either flame ionization or electron capture detectors. Chemicals were added to water in 500-mL flasks and gently shaken in a 25 ±1°C water bath until the concentration was constant. Samples of saturated water were centrifuged for 30 min.30 days old 0.2 g Pimephales promelas (Fathead Minnow) collected from Environmental Research Laboratory-Duluth. Fish were not fed during the test. Fish were randomly divided amongst the test tanks (control and five different concentrations each in duplicate for proportional diluter system and singly for continuous-flow minidilutor systems).Deaths were recorded after 1, 3, 6, 12, 24, 48,72, and 96 h. As the chemical was not highly soluble thus tested at 0.33 mg/l. After the exposure of chemical 1-Tridecanol on the Pimephales promelas (Fathead Minnow) for 96 hrs, no mortality were observed LC0 was 0.33 mg/l. Based on the no mortality at 0.33 mg/l chemical was consider as nontoxic at this concentration.

 

Similarly in the third study for the target chemical f supports the classification of chemical. In a 96 hours short team toxicity study, the effect of test material was evaluated on Fathead Minnows (Pimephales promelas). Test conducted on 30 days old fish. The results show no mortality in treated fish. Therefore, NOEC was considered to be 0.64 mg/L when Fathead Minnows (Pimephales promelas) treated with test material for 96 hours. The results show 0% Lethality i.e 100% survival for test organism’s at given concentration. Therefore, test material considered to be non-toxic at dose concentration 0.64 mg/L when tested on Pimephales promelas.

 

The fourth study consider for the test chemical . Aim of this study was to determine the nature and effect of test chemical when comes in contact with testfish. Test conducted according to the OECD Guideline 203 (Fish, Acute Toxicity Test). Test performed under the static condition for the total incubation period of 96 hrs. Based on the mortality of fish, because of the contact with test chemical , lethal concentration (LC50) was determine to be 2.8 mg/l after the exposure period of 96 hrs. Based on the LC50, chemical was consider as toxic and classified as aquatic acute 2 as per the CLP classification criteria.

 

 

Based on the experimental data as well as predicted data for the target chemical,it can be concluded that the substance is considered to be toxic to aquatic environment (fishes) and can be classified as aquatic acute 2 as per the criteria mentioned in CLP regulation.  

Short term toxicity to aq. invertebrates:

Based on the various experimental for the target chemical study have been reviewed to determine the toxic nature of test chemical on the mobility and growth of aquatic invertebrates. The studies are as mentioned below:

 In the first key study for the test chemical from authoritative database , determine the toxicity. Study was assess to evaluate the nature of test chemical chemical on the growth of aquatic invertebrates daphnia magna for the exposure of 96 hrs. Study was conducted according to the OECD Guideline 202 (Daphnia sp., Acute Immobilisation Test and Reproduction Test).Test performed under the semi-static condition for the total incubation period of 48 hrs. 100 mL/L vehicle were used for the preparation of 100 ml of test solution. Total amount of test solution was renewed after 24-hour exposure. Chemical tested at various concentrations 0, 0.10, 0.22, 0.46, 1.0, 2.2 mg/L (nominal concentrations) and also on vehicle (100 microl/l). 5 daphnia magna per vessel added and the test repeated 4 times. Room light, 16 hours light / 8 hours dark provided to the test organism.

 After the exposure of test chemical for 48 hrs effect were observed on the daphnia magna. After 24 hrs, the EC50 was 0.92 mg/l and after 48 hrs the NOEC was determine 0.22 mg/l. The EC50 was determine to be 0.61 mg/l with the 95 % CL of 0.49 - 0.75 mg/L. Based on the EC50, chemical was consider as toxic and classified as aquatic acute 1 and chronic 1 as per the CLP classification criteria.First experimental study was supported by the second supporting study from peer reviewed journal . Study was access to evaluate the toxic nature of test chemical on the growth of daphnia magna for 48 hrs of exposure. Study was conducted according to the OECD guideline 202 (Daphnia sp., Acute Immobilisation Test and Reproduction Test). After the exposure effect were observed. The effect concentration (EC50) was determine to be 0.5 mg/l by the test chemical . Based on the EC50 chemical was consider as toxic and can be consider to be classified as aquatic acute category 1 as per the CLP classification criteria.

 

Similarly in the third supporting study for the target chemical from peer reviewed journal . In a 48 hours short team toxicity study, the effect of test material was evaluated on Mysidopsis bahia (mysids). The test substance was introduced by feed in a concentration of 600 mg/L. The reactor was seeded with activated sludge from an industrial wastewater treatment plant treating ethoxylated surfactant. The reactors were operated for 10 weeks except reactor 4 of which operation was terminated after 8 weeks. The results show mortality at 2.2 mg/L. Therefore, LC50 for test material was considered to be 2.2 mg/L when tested on Mysidopsis bahia(mysids). Estimates by probit method and reported as concentration (for untreated product) or a dilettante (for effluents) resulting in death of 50 % of test organisms (LC50). LC50 for test material was determine to be 2.2 mg/l when tested on Mysidopsis bahia (mysids)  for 48 hours. Based on LC50 chemical was consider as toxic and classified as aquatic acute category 2, as per the CLP classification criteria.The data from the secondry data source for the target chemical concluded that the chemical was toxic to invertebrates.Study was assess to evaluate the nature of test chemical on the growth of aquatic invertebrates daphnia magna for the exposure of 96 hrs. As the chemical was treated with the test organism daphnia magna effect were observed (EC50) was determine at 0.51 mg/l. Based on the EC50, chemical was consider as toxic and classified as aquatic acute 1 as per the CLP classification criteria.Based on the experimental data of test chemical ,it can be concluded that the test substance is considered to be toxic and classified in aquatic acute 1 as per the CLP classification criteria.

 

Long term toxicity to invertebrates:

Study was assess to evaluate the long term effect of test chemical on the growth and reproduction Inhibition of aquatic invertebrates daphnia magna for the exposure of 21 days. Test conducted according to the OECD Guideline 211 (Daphnia magna Reproduction Test). 100 microL/L [mixed solution of Dimethylformamide / Hydrogenated castor oil (HCO-40) = 3/1 (w/w)] used as a vehicle. Test conducted under the Semi-static method at different concentrations control, vehicle control, 0.022, 0.046, 0.10, 0.22, 0.46 mg/L (geometric ratio: 2.2). 80 ml/vessel test solution added with the total amount of the test solution was renewed every 24 hours. 1 test organism added in each vessel and performed in the 10 replicates. After the exposure of test chemical with the aquatic invertebrates daphnia magna for 21 days, effect concentration EC50 was determine to be 0.46 mg/l and the NOEC was 0.22 mg/l. Based on the EC50 chemical was consider as toxic and can be consider to be classified as aquatic chronic 3 category as per the CLP classification criteria.The test substance is redialy biodegradable in aquatic environment and hence , based on that we can consider that the test substance is not classified as per CLP criteria.

 

Toxicity to aq. algae and cyanobacteria:

Based on the various experimental data for the target chemical study have been reviewed to determine the toxic nature on the growth of aquatic algae and cyanobacteria. The studies are as mentioned below:

 

In the first key study for the target chemical was described in authorative database,Study was assess to evaluate the nature and effect of test chemical on the growth of green algae for the exposure period of 72 hrs. Study was conducted under the static system. After the exposure effect was determine. Based on the growth rate inhibition of green algae due to the exposure of test chemical , the EC50 and NOEC was 0.012 mg/l and 0.0029 mg/l. But on the basis of Biomass and area under the growth curve, the EC50 and NOEC was determine to be 0.09 mg/l and 0.017 mg/l. Based on the EC50, test chemical was concluded as toxic and can be consider to be classified as aquatic acute 1 as per the CLP classification criteria.

 Similarly in the second supporting study for the target chemical from secondry source says that the chemical was toxic. Study was assess to evaluate the nature and effect of test chemical on the growth of algae for the exposure period of 72 hrs. After the exposure of chemical for 72 hrs, the EC50 was determine to be 0.1-1 mg/l. Based on the EC50, test chemical was concluded as toxic and can be consider to be classified as aquatic acute 1 and chronic 1 as per the CLP classification criteria.

 The fourth study consider for the test chemical from authorative database. Study was assess to evaluate the nature and effect of test chemical on the growth of green algae for the exposure period of 72 hrs. Study was conducted under the static system. After the exposure effect was determine. Based on the growth rate inhibition of green algae due to the exposure of test chemical , the EC50 and NOEC was 0.56 mg/l and 0.028 mg/l. But on the basis of Biomass and area under the growth curve, the EC50 and NOEC was determine to be 0.72 mg/l and 0.09 mg/l. Based on the EC50, test chemical was concluded as toxic and can be consider to be classified as aquatic acute 1 and chronic 1 as per the CLP classification criteria.

 Based on the experimental data for the test chemical it can be concluded that the substance is considered to be toxic to aquatic algae and cyanobacteria and classified as aquatic acute 1 and chronic 1 category as per the CLP classification criteria.

 

  Toxicity to microorganisms:

Various experimental data for the target chemical have been reviewed to determine the toxic nature of test substance on the growth of microorganisms. The studies are as mentioned below:

In the first key study for the target chemical from peer reviewed journal , Aim of this study was to determine the toxic nature of test chemical on the growth of microorganism Tetrahymena pyriformis for the total exposure period of 48 hrs. Chemical purchased from Aldrich Chemical Co., Milwaukee, Wisconsin having 95 % purity. Test performed in 3 replicates and each replicate was a five-step graded concentration series using freshly prepared stock solutions. Only replicate with control cultures in the late log-growth-phase (absorbance of 0.6 to 0.9) were used in these studies. Based on the growth rate inhibition of test organisms Tetrahymena pyriformis due to the exposure of test chemical for 48 hrs, the IGC50 was determine to be 586 mg/l.

First experimental study was supported by the second supporting study from peer reviewed journal. In a 48 hours short team toxicity study, the effect of test material was evaluated on 484 Tetrahymena pyriformis Ciliate. The test substance was test on the basis of QSAR in a concentration of 1001.5 mg/L (0.005mM/L). The results show 50% inhibition in growth rate of test organism’s population at given concentration. Therefore, IC50 for test material was considered to be growth inhibiter at 1001.5 mg/L (0.005mM/L) when tested on Tetrahymena pyriformis Ciliate for 48hrs.

 

Similarly in the third supporting study test chemical from another peer reviewed journal study was conducted. In a 48 hours short team toxicity study, the effect of test material was evaluated on 484 Tetrahymena pyriformis Ciliate. The test substance was test on the basis of QSAR, TOXICITY and BCF MODELS in a concentration of 1.05 mg/L (1050 µg/L). The results show 50% inhibition in growth rate of test organism’s population at given concentration. Therefore, IC50 for test material was considered to be growth inhibitor at 1.05 mg/L (1050 µg/L) when tested on 484 Tetrahymena pyriformis Ciliate for 2 days.

 

The first two study supports the nontoxic nature of chemical but according to the remaining study chemical was toxic and the MIC ranges from 1.05 mg/l to 1001.5 mg/l.