Registration Dossier

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from authoritative database
Justification for type of information:
Data is from authoritative database
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
Aim of this study was to determine the nature and effect of test chemical when comes in contact with Oryzias latipes.
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material: Tridecyl alcohol
- IUPAC name: 1-Tridecanol
- Molecular formula: C13H28O
- Molecular weight: 200.37 g/mol
- Smiles notation: C(CCCCCCO)CCCCCC
- InChl: 1S/C13H28O/c1-2-3-4-5-6-7-8-9-10-11-12-13-14/h14H,2-13H2,1H3
- Substance type: Organic
- Physical state: Solid
Analytical monitoring:
not specified
Vehicle:
not specified
Details on test solutions:
Details on test solutions
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Concentration of vehicle in test solution: 0.1 mL/L
Test organisms (species):
Oryzias latipes
Details on test organisms:
Details on test organisms
TEST ORGANISM
- Common name: Japanese rice fish (Medaka)
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Test temperature:
24 ± 1° C
Nominal and measured concentrations:
0, 1.0, 1.8, 3.2, 5.6, 10 mg/L
Details on test conditions:
Details on test conditions
TEST SYSTEM
- Material, size, headspace, fill volume: 5 L test solution was used
- Aeration: No
- Renewal rate of test solution (frequency/flow rate): Total amount of the test solution was renewed every 24 hours
- No. of organisms per vessel: 10 per concentration


OTHER TEST CONDITIONS
- Photoperiod: Room light, 16 hours light / 8 hours dark
Reference substance (positive control):
not specified
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
1.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: Other details not known
Validity criteria fulfilled:
not specified
Conclusions:
Based on the mortality of fish Oryzias latipes, because of the contact with test chemical , lethal concentration (LC50) was determine to be 1.7 mg/l after the exposure period of 96 hrs.
Executive summary:

Aim of this study was to determine the nature and effect of test chemical when comes in contact withOryzias latipes. Test conducted according to the OECD Guideline 203 (Fish, Acute Toxicity Test).

 

Test performed under the semi-static condition for the total incubation period of 96 hrs. 0.1 mL/L vehicle was used for the preparation of test solution. 5 L test solution were used in the study. Total amount of the test solution was renewed every 24 hours. Oryzias latipes (Japanese rice fish) is a freshwater fish. 10 fishes per concentration were tested with the tested solution as well as with control.

 

Based on the mortality of fish Oryzias latipes, because of the contact with chemical , test chemical lethal concentration (LC50) was determine to be 1.7 mg/l after the exposure period of 96 hrs. Based on the LC50, chemical was consider as toxic and classified as aquatic acute 2 as per the CLP classification criteria.

Description of key information

Aim of this study was to determine the nature and effect of test chemical when comes in contact withOryzias latipes. Test conducted according to the OECD Guideline 203 (Fish, Acute Toxicity Test).Test performed under the semi-static condition for the total incubation period of 96 hrs. 0.1 mL/L vehicle was used for the preparation of test solution. 5 L test solution were used in the study. Total amount of the test solution was renewed every 24 hours. Oryzias latipes (Japanese rice fish) is a freshwater fish. 10 fishes per concentration were tested with the tested solution as well as with control.Based on the mortality of fish Oryzias latipes, because of the contact with chemical , test chemical lethal concentration (LC50) was determine to be 1.7 mg/l after the exposure period of 96 hrs. Based on the LC50, chemical was consider as toxic and classified as aquatic acute 2 as per the CLP classification criteria.

Key value for chemical safety assessment

LC50 for freshwater fish:
1.7 mg/L

Additional information

Based on the various experimental and predicted data for the target chemical and experimental data for structurally and functionally similar read across chemicals study have been reviewed to determine the toxic nature of target chemical 1-Tridecanol (112-70-9) on the fishes. The studies are as mentioned below:

Aim of this study was to determine the nature and effect of test chemical when comes in contact withOryzias latipes. Test conducted according to the OECD Guideline 203 (Fish, Acute Toxicity Test).Test performed under the semi-static condition for the total incubation period of 96 hrs. 0.1 mL/L vehicle was used for the preparation of test solution. 5 L test solution were used in the study. Total amount of the test solution was renewed every 24 hours. Oryzias latipes (Japanese rice fish) is a freshwater fish. 10 fishes per concentration were tested with the tested solution as well as with control.Based on the mortality of fish Oryzias latipes, because of the contact with chemical , test chemical lethal concentration (LC50) was determine to be 1.7 mg/l after the exposure period of 96 hrs. Based on the LC50, chemical was consider as toxic and classified as aquatic acute 2 as per the CLP classification criteria.

 

First experimental study was supported by the second supporting study from peer reviewed journal.

Aim of this study was to determine the nature and effect of test chemical when comes in contact withpimephales promelas (Fathead minnow). Chemical was less soluble thus first extracted into hexane or hexane/ether (75: 25) and analyzed on an appropriate GC column utilizing either flame ionization or electron capture detectors. Chemicals were added to water in 500-mL flasks and gently shaken in a 25 ±1°C water bath until the concentration was constant. Samples of saturated water were centrifuged for 30 min.30 days old 0.2 g Pimephales promelas (Fathead Minnow) collected from Environmental Research Laboratory-Duluth. Fish were not fed during the test. Fish were randomly divided amongst the test tanks (control and five different concentrations each in duplicate for proportional diluter system and singly for continuous-flow minidilutor systems).Deaths were recorded after 1, 3, 6, 12, 24, 48,72, and 96 h. As the chemical was not highly soluble thus tested at 0.33 mg/l. After the exposure of chemical 1-Tridecanol on the Pimephales promelas (Fathead Minnow) for 96 hrs, no mortality were observed LC0 was 0.33 mg/l. Based on the no mortality at 0.33 mg/l chemical was consider as nontoxic at this concentration.

 

Similarly in the third study for the target chemical f supports the classification of chemical. In a 96 hours short team toxicity study, the effect of test material was evaluated on Fathead Minnows (Pimephales promelas). Test conducted on 30 days old fish. The results show no mortality in treated fish. Therefore, NOEC was considered to be 0.64 mg/L when Fathead Minnows (Pimephales promelas) treated with test material for 96 hours. The results show 0% Lethality i.e 100% survival for test organism’s at given concentration. Therefore, test material considered to be non-toxic at dose concentration 0.64 mg/L when tested on Pimephales promelas.

 

The fourth study consider for the test chemical . Aim of this study was to determine the nature and effect of test chemical when comes in contact with testfish. Test conducted according to the OECD Guideline 203 (Fish, Acute Toxicity Test). Test performed under the static condition for the total incubation period of 96 hrs. Based on the mortality of fish, because of the contact with test chemical , lethal concentration (LC50) was determine to be 2.8 mg/l after the exposure period of 96 hrs. Based on the LC50, chemical was consider as toxic and classified as aquatic acute 2 as per the CLP classification criteria.

 

 

Based on the experimental data as well as predicted data for the target chemical,it can be concluded that the substance is considered to be toxic to aquatic environment (fishes) and can be classified as aquatic acute 2 as per the criteria mentioned in CLP regulation.