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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Data is from authoritative database
Justification for type of information:
Data is from authoritative database
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Principles of method if other than guideline:
Study was assess to evaluate the nature of test chemical on the growth of aquatic invertebrates daphnia magna for the exposure of 96 hrs.
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material: Tridecyl alcohol
- IUPAC name: 1-Tridecanol
- Molecular formula: C13H28O
- Molecular weight: 200.37 g/mole
- Smiles notation: C(CCCCCCO)CCCCCC
- InChl: 1S/C13H28O/c1-2-3-4-5-6-7-8-9-10-11-12-13-14/h14H,2-13H2,1H3
- Substance type: Organic
- Physical state: Solid
Analytical monitoring:
not specified
Vehicle:
yes
Details on test solutions:
Details on test solutions
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Concentration of vehicle in test solution: 100 microL/L
Test organisms (species):
Daphnia magna
Details on test organisms:
Details on test organisms
TEST ORGANISM
- Common name: Water flea
Test type:
semi-static
Water media type:
freshwater
Total exposure duration:
48 h
Test temperature:
24 ± 1° C
Nominal and measured concentrations:
0, 0.10, 0.22, 0.46, 1.0, 2.2 mg/L (nominal concentrations) and also on vehicle (100 microl/l)
Details on test conditions:
Details on test conditions
TEST SYSTEM
- Material, size, headspace, fill volume: test solution volume 100 mL
- Renewal rate of test solution (frequency/flow rate): Total amount of the test solution was renewed every 24 hours
- No. of organisms per vessel: 5 per vessel (20 per concentration)
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4


OTHER TEST CONDITIONS
- Photoperiod: Room light, 16 hours light / 8 hours dark
Reference substance (positive control):
not specified
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
0.61 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
not specified
Remarks on result:
other: 95 % CL: 0.49 - 0.75 mg/L

1) Results after 24-hour exposure:

- 50 % Effective concentration (EC50) for immobilization: 0.92 mg/L ( 95 % CL: 0.75 - 1.1 mg/L)

2) Results after 48-hour exposure:

- No observed effect concentration (NOEC) for immobilization: 0.22 mg/L,

- Lowest concentration causing 100 % immobility: 2.2 mg/L

Validity criteria fulfilled:
not specified
Conclusions:
After the exposure of test chemical chemical for 48 hrs effect were observed on the daphnia magna. The EC50 was determine to be 0.61 mg/l with the 95 % CL of 0.49 - 0.75 mg/L.
Executive summary:

Study was assess to evaluate the nature of test chemical chemical on the growth of aquatic invertebrates daphnia magna for the exposure of 96 hrs. Study was conducted according to the OECD Guideline 202 (Daphnia sp., Acute Immobilisation Test and Reproduction Test).

Test performed under the semi-static condition for the total incubation period of 48 hrs. 100 mL/L vehicle were used for the preparation of 100 ml of test solution. Total amount of test solution was renewed after 24-hour exposure. Chemical tested at various concentrations 0, 0.10, 0.22, 0.46, 1.0, 2.2 mg/L (nominal concentrations) and also on vehicle (100 microl/l). 5 daphnia magna per vessel added and the test repeated 4 times. Room light, 16 hours light / 8 hours dark provided to the test organism.

 

After the exposure of test chemical for 48 hrs effect were observed on the daphnia magna. After 24 hrs, the EC50 was 0.92 mg/l and after 48 hrs the NOEC was determine 0.22 mg/l. The EC50 was determine to be 0.61 mg/l with the 95 % CL of 0.49 - 0.75 mg/L. Based on the EC50, chemical was consider as toxic and classified as aquatic acute 1 and chronic 1 as per the CLP classification criteria.

Description of key information

Study was assess to evaluate the nature of test chemical chemical on the growth of aquatic invertebrates daphnia magna for the exposure of 96 hrs. Study was conducted according to the OECD Guideline 202 (Daphnia sp., Acute Immobilisation Test and Reproduction Test).

Test performed under the semi-static condition for the total incubation period of 48 hrs. 100 mL/L vehicle were used for the preparation of 100 ml of test solution. Total amount of test solution was renewed after 24-hour exposure. Chemical tested at various concentrations 0, 0.10, 0.22, 0.46, 1.0, 2.2 mg/L (nominal concentrations) and also on vehicle (100 microl/l). 5 daphnia magna per vessel added and the test repeated 4 times. Room light, 16 hours light / 8 hours dark provided to the test organism.

 

After the exposure of test chemical for 48 hrs effect were observed on the daphnia magna. After 24 hrs, the EC50 was 0.92 mg/l and after 48 hrs the NOEC was determine 0.22 mg/l. The EC50 was determine to be 0.61 mg/l with the 95 % CL of 0.49 - 0.75 mg/L. Based on the EC50, chemical was consider as toxic and classified as aquatic acute 1 as per the CLP classification criteria.

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
0.92 mg/L

Additional information

Based on the various experimental for the target chemical study have been reviewed to determine the toxic nature of test chemical on the mobility and growth of aquatic invertebrates. The studies are as mentioned below:

 

In the first key study for the test chemical from authoritative database , determine the toxicity. Study was assess to evaluate the nature of test chemical chemical on the growth of aquatic invertebrates daphnia magna for the exposure of 96 hrs. Study was conducted according to the OECD Guideline 202 (Daphnia sp., Acute Immobilisation Test and Reproduction Test).Test performed under the semi-static condition for the total incubation period of 48 hrs. 100 mL/L vehicle were used for the preparation of 100 ml of test solution. Total amount of test solution was renewed after 24-hour exposure. Chemical tested at various concentrations 0, 0.10, 0.22, 0.46, 1.0, 2.2 mg/L (nominal concentrations) and also on vehicle (100 microl/l). 5 daphnia magna per vessel added and the test repeated 4 times. Room light, 16 hours light / 8 hours dark provided to the test organism.

 

After the exposure of test chemical for 48 hrs effect were observed on the daphnia magna. After 24 hrs, the EC50 was 0.92 mg/l and after 48 hrs the NOEC was determine 0.22 mg/l. The EC50 was determine to be 0.61 mg/l with the 95 % CL of 0.49 - 0.75 mg/L. Based on the EC50, chemical was consider as toxic and classified as aquatic acute 1 and chronic 1 as per the CLP classification criteria.

 

First experimental study was supported by the second supporting study from peer reviewed journal . Study was access to evaluate the toxic nature of test chemical on the growth of daphnia magna for 48 hrs of exposure. Study was conducted according to the OECD guideline 202 (Daphnia sp., Acute Immobilisation Test and Reproduction Test). After the exposure effect were observed. The effect concentration (EC50) was determine to be 0.5 mg/l by the test chemical . Based on the EC50 chemical was consider as toxic and can be consider to be classified as aquatic acute category 1 as per the CLP classification criteria.

 

 

Similarly in the third supporting study for the target chemical from peer reviewed journal . In a 48 hours short team toxicity study, the effect of test material was evaluated on Mysidopsis bahia (mysids). The test substance was introduced by feed in a concentration of 600 mg/L. The reactor was seeded with activated sludge from an industrial wastewater treatment plant treating ethoxylated surfactant. The reactors were operated for 10 weeks except reactor 4 of which operation was terminated after 8 weeks. The results show mortality at 2.2 mg/L. Therefore, LC50 for test material was considered to be 2.2 mg/L when tested on Mysidopsis bahia(mysids). Estimates by probit method and reported as concentration (for untreated product) or a dilettante (for effluents) resulting in death of 50 % of test organisms (LC50). LC50 for test material was determine to be 2.2 mg/l when tested on Mysidopsis bahia (mysids)  for 48 hours. Based on LC50 chemical was consider as toxic and classified as aquatic acute category 2, as per the CLP classification criteria

 

The data from the secondry data source for the target chemical concluded that the chemical was toxic to invertebrates.

Study was assess to evaluate the nature of test chemical on the growth of aquatic invertebrates daphnia magna for the exposure of 96 hrs. As the chemical was treated with the test organism daphnia magna effect were observed (EC50) was determine at 0.51 mg/l. Based on the EC50, chemical was consider as toxic and classified as aquatic acute 1 as per the CLP classification criteria.

 

Based on the experimental data of test chemical ,it can be concluded that the test substance is considered to be toxic and classified in aquatic acute 1 as per the CLP classification criteria.