2,2'-oxydiethanol

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Pharmacokinetics and biotransformation of diethylene glycol and ethylene glycol in the rat.

Author:

Lenk W, Löhr D, Sonnenbichler J

Year:

1989

Bibliographic source:

Xenobiotica 19: 961-979

Materials and methods

Principles of method if other than guideline:

The authors report results on the pharmacokinetics and metabolism of DEG and EG in rat, obtained with 14C-labelled DEG and non-labelled EG, using high-resolution NMR spectroscopy as a highly specific and rapid technique.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

2,2'-oxydiethanol

EC Number:

203-872-2

EC Name:

2,2'-oxydiethanol

Cas Number:

111-46-6

Molecular formula:

C4H10O3

IUPAC Name:

2-(2-hydroxyethoxy)ethan-1-ol

Specific details on test material used for the study:

- Name of test material (as cited in study report): Diethylene glycol
- Analytical purity: > 99%
- Other: purchased from Fluka AG, Switzerland

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 250 - 300 g
- Housing: in stainless-steel metabolic cages
- Diet: Alma standard diet for rats and mice (H 1003, Kempten, Germany), ad libitum
- Water: ad libitum

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

physiological saline

Details on oral exposure:

Diethylene glycol (DEG) was administered to rats by gavage as 50% solution in 0.9% saline (v/v), at doses of 1 (i.e., 1.12 g), 5, 10, 12.5, 15 and 17. 5 mL DEG/kg bw.

Doses:

1 (i.e., 1.12 g), 5, 10, 12.5, 15 and 17. 5 mL DEG/kg bw.

No. of animals per sex per dose:

10

Control animals:

no

Details on study design:

Observation period: 7 days

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LD50

Effect level:

19 600 mg/kg bw

Based on:

test mat.

Clinical signs:

other: The following effects have developed consecutively: narcotic phase, diuretic phase and thirst, drop of the pH of the urine and blood, either recovery or hydrotropic degeneration of the renal tubules and anuria, accumulation of urea and uric acid in the bl