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Ecotoxicological information

Toxicity to microorganisms

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Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: The test parameters evaluated in this study comply with an in-house protocol that is expected to be robust, well documented and scientifically acceptable.
Qualifier:
according to guideline
Guideline:
other: BASF in-house protocol
Principles of method if other than guideline:
Growth inhibition was evalated for 17 hours in the bacteria Pseudomonas putida.
GLP compliance:
not specified
Analytical monitoring:
not specified
Test organisms (species):
Pseudomonas putida
Test type:
static
Water media type:
freshwater
Total exposure duration:
17 h
Hardness:
Not reported
Test temperature:
20
pH:
Not reported
Dissolved oxygen:
Not reported
Salinity:
Not reported
Nominal and measured concentrations:
Not reported
Duration:
17 h
Dose descriptor:
EC10
Effect conc.:
ca. 177 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Duration:
17 h
Dose descriptor:
EC50
Effect conc.:
ca. 281 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Duration:
17 h
Dose descriptor:
other: EC90
Effect conc.:
ca. 544 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Conclusions:
For the bacteria Pseudomonas putida, the 17-hour EC10, EC50 and EC90 for growth inhibition are 177, 281 and 544 mg MEKO/L, respectively.
Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
disregarded due to major methodological deficiencies
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: This study does not provide sufficient experimental details and was not conducted according to GLPs
Qualifier:
no guideline available
Guideline:
other:
GLP compliance:
no
Details on test solutions:
The concentration at which effects still occured was estimated by a dilution series.
Test organisms (species):
Pseudomonas sp.
Test type:
not specified
Water media type:
freshwater
Dose descriptor:
LOEC
Effect conc.:
>= 630 mg/L
Nominal / measured:
not specified
Conc. based on:
not specified
Basis for effect:
not specified
Conclusions:
For a Pseudomonas sp., the LOEC for an effect is estimated to be >630 mg MEKO/L. The basis for effect, duration of exposure, and the other MEKO concentrations tested are not known.

Description of key information

The 17-h EC10 for Pseudomonas putida of 177 mg/L will be used in the assessment.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:
177 mg/L

Additional information

In a static freshwater micro-organism toxicity study with Pseudomonas putida (BASF Ecotoxicology Laboratory, 1988), performed according to BASF protocols, the 17-h EC10 is 177 mg/L and the 17-h EC50 is 281 mg/L, based on growth inhibition. No data are available on the GLP status and analytical monitoring. In another freshwater study with Pseudomonas sp., for which only information from secondary sources is available (Meinck et al., 1968) a LOEC of = 630 mg/L is reported. The value from the study by BASF Ecotoxicology Laboratory (1988) will be used in the assessment as the results are more critical and results from secondary sources cannot be used.