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Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1991
Report date:
1991

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 402 (Acute Dermal Toxicity)
Deviations:
yes
Remarks:
only tested up to 1000 mg/kg bw
GLP compliance:
yes
Test type:
standard acute method
Limit test:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Butanone oxime
EC Number:
202-496-6
EC Name:
Butanone oxime
Cas Number:
96-29-7
Molecular formula:
C4H9NO
IUPAC Name:
butan-2-one oxime
Test material form:
liquid

Test animals

Species:
rabbit
Strain:
New Zealand White
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Mohican Valley Rabbitry, Loudonville, OH
- Weight at study initiation: 1.8-2.3 kg
- Fasting period before study: none
- Housing: suspended stainless steel cages
- Diet (e.g. ad libitum): Agway Prolab Rabbit Feed, ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: at least 5 days


ENVIRONMENTAL CONDITIONS
- Room: environmentally controlled room
- Photoperiod (hrs dark / hrs light): 12/12


Administration / exposure

Type of coverage:
occlusive
Vehicle:
unchanged (no vehicle)
Details on dermal exposure:
MEKO was administered to one group of five male and five female rabbits by a single dermal dose at a level of 1000 mg/kg.

On the day prior to dosing, the fur was clipped from the dorsal area of the trunk of each animal using small animal clippers. The clipped area measured approximately 20 cm x 12 cm and constituted approximately 10% of the animal's total body surface. Care was taken during clipping to avoid accidental abrasion to the skin. On the following day, 5 male and 5 female rabbits were weighed and MEKO was applied uniformly over the clipped area at a dose level of 1000 mg/kg. Each animal's dose was contained at the area of application using an 8-ply gauze dressing covered with plastic wrap. A tubular stockinette sleeve was positioned around the trunk of each animal and secured in place using tape. After an exposure period of 24 hours, the stockinette sleeve, plastic wrap and gauze dressing were removed. The exposure site on each animal was rinsed with distilled water to remove residual MEKO. No attempt was made to quantitate the amount of nonabsorbed MEKO.
Duration of exposure:
24 hours
Doses:
1000 mg/kg bw
No. of animals per sex per dose:
5/sex/dose
Control animals:
no

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
> 1 000 mg/kg bw
Based on:
test mat.
Mortality:
No mortality occurred in a 14 day period after dosing.
Body weight:
All animals gained weight during the study.

Applicant's summary and conclusion

Interpretation of results:
other: CLP/EU GHS criteria not met, no classification required according to Regulation (EC) No 1272/2008
Conclusions:
The acute dermal LD50 of methyl ethyl ketoxime in rabbits is estimated to be greater than 1000 mg/kg body weight.