Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 202-496-6 | CAS number: 96-29-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: The test parameters evaluated in this study comply with a specific test guideline (EG-Richtlinie 79/831/EWG, Anhang V, C2), are well documented, scientifically acceptable, but not performed according to GLPs.
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- no
- Analytical monitoring:
- no
- Details on test solutions:
- Concentration tested was 500 mg/L.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- 5 daphnia/vessel (10 ml). 4 vessels per test concentration.
- Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Test temperature:
- 20 Degrees centigrade.
- pH:
- About 8.0 during testperiod.
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- <= 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- >= 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- >= 500 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Conclusions:
- For the freshwater crurstacean Daphnia magna, the EC0, EC50 and EC100 (mobility) are estimated to be equal to 500, >500, and >500 mg MEKO/L, respectively.
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was conducted according to generally vaiid procedures and according to GLP guidelines. All parameters described are closely related or comparable to guideline methods.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations:all
- Sampling method:test item concentrations were measured using the solution mixed with equal volume taken out from the middle layer of the test solution in four test vessels in each exposure level - Details on test solutions:
- Nominal concentrations of MEKO were 1000, 556, 309, 171, 95.3 or 52.9 mg/L. A laboratory water control was included. A solubilizer was not used. Concentrations of the test substance were kept close to the nominal concentrations (99.5 to 106%).
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- 20 daphnids (4 replicates; 5 organisms per replicate) were exposed to various nominal concentrations of MEKO.
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 48 h
- Hardness:
- 40.5 mg/L (as CaC03)
- Test temperature:
- 20±1°C
- pH:
- 7.6
- Details on test conditions:
- TEST SYSTEM
- Test vessel:Petri dish (inside diameter 8.5 cm, depth 5.7 cm)
- Material, size, headspace, fill volume:
- No. of organisms per vessel:5
- No. of vessels per concentration (replicates):4
- No. of vessels per control (replicates):4
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod:16-hour light/8-hour dark
- Light intensity:Artificial light of white fluorescent lamp
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
TEST CONCENTRATIONS
- Spacing factor for test concentrations:geometric series with a factor 1.8
- Justification for using less concentrations than requested by guideline:
- Range finding study: 30% immobility at 24 hours, 100% immobility at 48 hours in 500 mg/L; no immobilization at 62.5 mg/l
- Test concentrations:52.9, 95.3, 171, 309, 556, 1000 - Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- ca. 201 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- ca. 93 mg/L
- Nominal / measured:
- nominal
- Details on results:
- - Mortality of control: none
- Effect concentrations exceeding solubility of substance in test medium: none - Validity criteria fulfilled:
- yes
- Conclusions:
- For the freshwater crustacean Daphnia magna, the 48-hour EC50 (mobility) is 201 mg MEKO/mL.
Referenceopen allclose all
The ratio of the measured concentration to the nominal value ranged from 103 to 106% at the start of the exposure, and from 99.5 to 104% at the end of the exposure
Description of key information
The 48-h EC50 of 201 mg/L in Daphnia magna will be used in the assessment.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 201 mg/L
Additional information
Two short-term toxicity tests with Daphnia magna are available for MEKO. In the first one, a GLP-compliant static OECD Guideline 202 test with analytical monitoring, a 48-h EC50 of ca. 201 mg/L is reported (Kurume Laboratory, 1998c). In the second study, performed without GLP according to EU Method C2, a 48-h LC50 of = 500 mg/L is reported based on mobility (BASF, 1989). The most critical study of Kurume Laboratory (1998c) is regarded as the key study.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.