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Ecotoxicological information

Toxicity to aquatic plants other than algae

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Administrative data

Endpoint:
toxicity to aquatic plants other than algae
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Not a guideline study, but reasonably documented and performed.

Data source

Reference
Reference Type:
publication
Title:
Duckweed Lemna minor as a Tool for Testing Toxicity of Coal Residues and Polluted Sediments
Author:
Jenner HA, Janssen-Mommen JPK
Year:
1993
Bibliographic source:
Archieves of the Environmental Contamination and Toxicology

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Toxicity to growth of aquatic plants based on Rombach, 1976.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Assessment was based on As (III)

Sampling and analysis

Analytical monitoring:
yes

Test organisms

Test organisms (species):
Lemna minor

Study design

Test type:
flow-through
Water media type:
freshwater
Total exposure duration:
14 d

Test conditions

Test temperature:
23 °C
pH:
5
Nominal and measured concentrations:
Depending on As concentrations in the samples, elements in growth medium were measured by atomic absorption spectrophotometry (AAS) or by inductively coupled plasma (ICP).
Details on test conditions:
Test medium: Gorham medium (1950) modified by Rombach (1976)

As 2.5 micromol of EDTA were added to the growth medium, EDTA was not assumed to affect toxicity of the As anion as according to Rahman et al (2007) the addition of EDTA to the culture media increases the uptake of the As(V) or As(III) into plant tissue.

Rahman et al (2007) Influence of EDTA and chemical species on arsenic accumulation in Spirodela polyrhiza L. (duckweed). Ecotoxicol Environ Saf. 70, 311-318

Results and discussion

Effect concentrations
Duration:
14 d
Dose descriptor:
EC50
Effect conc.:
0.63 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
dissolved
Basis for effect:
other: growth as leaf coverage
Details on results:
The reported NOEC value of < 0.75 mg/L was not considered because the NOEC > EC50.

Applicant's summary and conclusion

Conclusions:
The EC50 value reported for freshwater plants is 0.63 mg As/L for the growth (i.e. leaf coverage) of Lemna minor.