Registration Dossier

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study, well documented and well performed according to scientific standards.

Data source

Reference
Reference Type:
publication
Title:
Acute and chronic effects of water quality criteria-based metal mixtures on three aquatic species
Author:
Spehar RL, Fiandt JT
Year:
1986
Bibliographic source:
Environmental Toxocology and Chemistry 5, 917-931

Materials and methods

Test guidelineopen allclose all
Qualifier:
equivalent or similar to
Guideline:
other: American Society for Testing and Materials(ASTM), 1980 Standard practice for conducting acute toxicity tests with fishes, macroinvertebrates and amphibians. E729-80
Qualifier:
equivalent or similar to
Guideline:
other: American Public Health Association, American Water Works Association and Water Pollution Control Federation. 1985. Standard methods for the Examination of Water and Waste Water, 16th. ed.
Qualifier:
equivalent or similar to
Guideline:
other: US. Environmental Protection Agency. 1979. Methods for chemical analysis of water and wastes. EPA 600-479-020. Environmental Monitoring and Support Laboratory, Office of Research and Development
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test organisms

Test organisms (species):
Pimephales promelas
Details on test organisms:
average age: 30 d; average weight: 0.15 g

Study design

Test type:
flow-through
Water media type:
freshwater
Total exposure duration:
4 d

Test conditions

Hardness:
43.9 mg/L CaCO3
Test temperature:
25 °C
pH:
7.4 (6.0-8.1)
Dissolved oxygen:
at least 70 % saturation
Nominal and measured concentrations:
Test concentrations were analytically confirmed.

Results and discussion

Effect concentrations
Duration:
4 d
Dose descriptor:
LC50
Effect conc.:
12.6 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
dissolved
Basis for effect:
mortality
Remarks on result:
other: CL: 9.90-15.90 mg/L

Applicant's summary and conclusion

Conclusions:
The 4-d LC50 for mortality of the fish Pimephales promelas is 12.6 mg As/L, and this LC50 value is considered for the Chemicals Safety Assessment.