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Ecotoxicological information

Toxicity to other aquatic organisms

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Administrative data

Endpoint:
toxicity to other aquatic vertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Chronic Exposure to Pentavalent Arsenic of Larval Leopard Frogs (Rana pipiens): Bioaccumulation and Reduced Swimming Performance
Author:
Chen TH, Gross JA, Karasov WH
Year:
2009
Bibliographic source:
Ecotoxicology 18, 587-593

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
long-term toxicity of amphibia
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Specific details on test material used for the study:
HNa2AsO47H2O, Alfa Aesar, Ward Hill, MA, USA

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Water samples were drawn at time zero and 48 h later just before water was changed in the tanks.

Test solutions

Details on test solutions:
Appropriate volumes of the stock solution (B1 ml) were used to
dose the tanks (containing 18 l of dechlorinated, ultraviolet
radiation-sterilized water) for the As treatments

Test organisms

Aquatic vertebrate type (other than fish):
frog
Test organisms (species):
Rana pipiens
Details on test organisms:
larval northern leopard frog (Rana pipiens) from early developmental stage
purchased from Nasco (Fort Atkinson, WI, USA)

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
113 d

Test conditions

Hardness:
170 mg/L CaCO3
Test temperature:
22–23°C
pH:
7.9
Nominal and measured concentrations:
nominal concentrations: control, 10, 20, 150, 500 and 1000 µg As/L
measured concentrations: 1.4 ± 0.1, 13.5 ± 0.2, 27.7 ± 1.4, 154.1 ± 4.8, 514.0 ± 3.4, and 1,026.2 ± 1.7 µg As/L
Details on test conditions:
During the entire experimental period the water temperature was maintained at 22–23°C and light: dark cycle was constant at 14:10 h.

Results and discussion

Effect concentrationsopen allclose all
Duration:
44 d
Dose descriptor:
NOEC
Effect conc.:
>= 1 026 µg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
element (dissolved fraction)
Remarks:
As
Basis for effect:
mortality
Duration:
44 d
Dose descriptor:
NOEC
Effect conc.:
>= 1 026 µg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
element (dissolved fraction)
Remarks:
As
Basis for effect:
other:
Remarks:
growth rate (length)
Duration:
44 d
Dose descriptor:
NOEC
Effect conc.:
>= 1 026 µg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
element (dissolved fraction)
Remarks:
As
Basis for effect:
other: development (metamorphosis)

Applicant's summary and conclusion

Conclusions:
A long-term NOEC value of ≥ 1026 mg As/L is reported for mortality, growth and development of the freshwater amphibian species Rana pipiens.