Diarsenic trioxide

Toxicity to other aquatic organisms

Administrative data

Endpoint:

toxicity to other aquatic vertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Chronic Exposure to Pentavalent Arsenic of Larval Leopard Frogs (Rana pipiens): Bioaccumulation and Reduced Swimming Performance

Author:

Chen TH, Gross JA, Karasov WH

Year:

2009

Bibliographic source:

Ecotoxicology 18, 587-593

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

long-term toxicity of amphibia

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

10048-95-0

Cas Number:

10048-95-0

IUPAC Name:

10048-95-0

Specific details on test material used for the study:

HNa2AsO47H2O, Alfa Aesar, Ward Hill, MA, USA

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Water samples were drawn at time zero and 48 h later just before water was changed in the tanks.

Test solutions

Details on test solutions:

Appropriate volumes of the stock solution (B1 ml) were used to
dose the tanks (containing 18 l of dechlorinated, ultraviolet
radiation-sterilized water) for the As treatments

Test organisms

Aquatic vertebrate type (other than fish):

frog

Test organisms (species):

Rana pipiens

Details on test organisms:

larval northern leopard frog (Rana pipiens) from early developmental stage
purchased from Nasco (Fort Atkinson, WI, USA)

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

113 d

Test conditions

Hardness:

170 mg/L CaCO3

Test temperature:

22–23°C

pH:

7.9

Nominal and measured concentrations:

nominal concentrations: control, 10, 20, 150, 500 and 1000 µg As/L
measured concentrations: 1.4 ± 0.1, 13.5 ± 0.2, 27.7 ± 1.4, 154.1 ± 4.8, 514.0 ± 3.4, and 1,026.2 ± 1.7 µg As/L

Details on test conditions:

During the entire experimental period the water temperature was maintained at 22–23°C and light: dark cycle was constant at 14:10 h.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

44 d

Dose descriptor:

NOEC

Effect conc.:

>= 1 026 µg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

element (dissolved fraction)

Remarks:

As

Basis for effect:

mortality

Effect concentrations 2

Duration:

44 d

Dose descriptor:

NOEC

Effect conc.:

>= 1 026 µg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

element (dissolved fraction)

Remarks:

As

Basis for effect:

other:

Remarks:

growth rate (length)

Effect concentrations 3

Duration:

44 d

Dose descriptor:

NOEC

Effect conc.:

>= 1 026 µg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

element (dissolved fraction)

Remarks:

As

Basis for effect:

other: development (metamorphosis)

Applicant's summary and conclusion

Conclusions:

A long-term NOEC value of ≥ 1026 mg As/L is reported for mortality, growth and development of the freshwater amphibian species Rana pipiens.