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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Cited experiment was summarized in the National Toxicology Program-Center for the Evaluation of Risks to Human Reproduction Monograph on the Potential Human Reproductive and Developmental Effects of 2-Bromopropane; original study was not reviewed
Cross-reference
Reason / purpose:
reference to same study

Data source

Reference
Reference Type:
review article or handbook
Title:
Unnamed
Year:
2003
Report Date:
2003

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Reference
Name:
Unnamed
Type:
Constituent

Method

Target gene:
Histidine operon
Test concentrations with justification for top dose:
Preliminary (50-5,000 ug/ plate) and secondary (313-5,000 ug/plate)

Results and discussion

Any other information on results incl. tables

A dose-related increase in mutations was noted with 2-bromopropane treatment in strain TA100 with S9 activation and in TA1535 with and without activation. Results in E. coli and the other Salmonella strains were negative. The authors stated that mutation in strains TA100 and TA1535 indicate base-pair substitutions.

There was a mutagenic effect in two strains of Salmonella, one only with metabolic activation, one with and without metabolic activation. The two strains both detect base-pair substitutions, raising the likelihood that 2-bromopropane, at least under some circumstances, is mutagenic.

Applicant's summary and conclusion

Conclusions:
In this supporting study, dose-related increases in mutations were noted with 2-bromopropane treatment in S. typhimurium strain TA100 with S9 activation and in TA1535 with and without activation. These results indicated that 2-bromopropane is mutagenic under certain conditions.