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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Partial report available. Report states that the study meets all criteria for reliability, but not all information could be verified.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report Date:
1995

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
Version / remarks:
also acc. to OECD 301 D
Deviations:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent

Study design

Oxygen conditions:
aerobic
Duration of test (contact time):
28 d
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Reference substance
Reference substance:
benzoic acid, sodium salt

Results and discussion

Test performance:
The criteria for test validity have been met:
The difference between the highest and lowest values of replicates obtained in the test series (Series 2) is less than 20%;
The percentage of degradation of reference substance in the series inoculum control (Series 4) is greater than 60% in 14 days;
The degradation of reference substance in the inhibition control (Series 3) exceeds 25% during the first 14 days;
the degradation of reference substance has not been inhibited by the presence of the test substance;
The loss of oxygen in blank (Series 1) (does not contain inoculum) exceeded 1.5 mg/l of dissolved oxygen after 28 days;
The residual oxygen concentration in the test flasks has always been greater than 0.5 mg/l throughout the duration of the test.
% Degradation
Parameter:
% degradation (O2 consumption)
Value:
68.6
Sampling time:
21 d
Remarks on result:
other: plateau @ 21 d
Details on results:
The substance showed no inhibitory effect vis-a-vis micro-organisms. The criteria for a valid test have been met: The difference between the
highest and lowest values of replicates obtained in the test series (Series 2) is less than 20%.

BOD5 / COD results

Results with reference substance:
The percentage of degradation of reference substance is greater than 60% in 14 days; The degradation of reference substance has not been inhibited by the presence of the test substance.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
readily biodegradable
Conclusions:
Under the conditions of the test, the percent biodegradation of 2-bromo -Propane was 68.6% in 21 days (plateau). Ten days following the beginning of the test, it reached approximately 56% biodegradation. The substance may be considered "readily biodegradable" according to the criteria in Guideline 301 of the OECD.
Executive summary:
The biodegradability of 2 -bromopropane was conducted using procedures set forth in Annex C.4-E to Directive 92/69/EEC. This protocol is in agreement with the OECD Guideline No. 301 D adopted 17/7/92. RESULTS SYNOPSIS*:Under the conditions of the test, the percent biodegradation of 2-bromo -Propane was 68.6% in 21 days (plateau). Ten days following the beginning of the test,it has reached about 56% biodegradation. The substance may be considered "readily biodegradable" according to the criteria in Guideline 301 of the OECD. *Partial report available. Report states that the study meets all criteria for reliability, but not all information can be verified.