Registration Dossier

Ecotoxicological information

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
Results should be interpreted with caution because the test substance concentration was not maintained above 80% of the initial concentration. Study summary in English available for review only. Full study report not available in English; therefore, not all information was verified.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2001
Report Date:
2001

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 211 (Daphnia magna Reproduction Test)
Deviations:
no
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d

Test conditions

Hardness:
See Table 11, in 'Attached Background Material"
Test temperature:
See Table 8, in 'Attached Background Material"
pH:
See Table 10, in 'Attached Background Material"
Dissolved oxygen:
See Table 9, in 'Attached Background Material"
Nominal and measured concentrations:
See Table 1-1 and 1-2, in 'Attached Background Material"
Nominal concentrations: control, 1.0, 2.7, 7.1, 19.0, and 50.0 mg/L (geometric ratio approx: 2.7)
Details on test conditions:
TEST SYSTEM
- Type: Covered the surface of the solutions by teflon seat.
- Fill volume: 80 mL/vessel
- Renewal rate of test solution (frequency/flow rate): All test solutions exchanged every 24 hours
- No. of organisms per vessel: 10/concentration (1/vessel)
- No. of vessels per concentration (replicates): 10 vessels/concentration
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 Medium
OTHER TEST CONDITIONS
- Photoperiod: 16 hrs light/8 hrs dark
- Test concentrations: Nominal concentrations: control, 1.0, 2.7, 7.1, 19.0, and 50.0 mg/L (geometric ratio approx: 2.7)

Results and discussion

Effect concentrationsopen allclose all
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
13.4 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
test mat.
Basis for effect:
reproduction
Remarks on result:
other: 95% C.I.: 12.3 mg/L to 14.9 mg/L
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
4.94 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
test mat.
Basis for effect:
reproduction
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
12 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
test mat.
Basis for effect:
reproduction
Details on results:
See Table 2-1, 2-2, 3, 4, 5, 6, and 7, in 'Attached Background Material"

Any other information on results incl. tables

See Table 2-1, 2-2, 3, 4, 5, 6, and 7, and Figure 1 and 2, in 'Attached Background Material". Parental Daphnia median lethal concentration (LC50) > 33.5 mg/L.

The LC50 value and its 95% confidence limits could not be determined by statistical method because the mortality of parental Daphnia at the maximum concentration level was less than 50%.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 21 day EC50 based on reproduction prohibition was 13.4 mg/L.  The 21-day NOEC was 4.94 mg/L. The 21-day LOEC was 12.0 mg/L. EC50, NOEC, and LOEC based on time-weighted mean measured concentrations. Results should be interpreted with caution because the test substance concentration was not maintained above 80% of the initial concentration. Study summary in English available for review only. Full study report not available in English; therefore, not all information could be verified.
Executive summary:

The 21-day toxicity of 2 -bromopropane to Daphnia magna was studied under semi-static conditions in accordance with the OECD Guidelines for the Testing of Chemicals, No. 211, Daphnia magna Reproduction Test. Daphnids were exposed to

2 -bromopropane at nominal concentration of controla, 1.0, 2.7, 7.1, 19.0 and 50.0 mg/L (geometric ratio approximately: 2.7). The 21 -da EC50 based on reproduction was 13.4 mg/L. The 21- day NOEC was 4.94 mg/L.

The 21-day LOEC was 12.0 mg/L. Results should be interpreted with caution because the test substance concentration was not maintained above 80% of the initial concentration. However, the EC50, NOEC, and LOEC are based on time-weighted mean measured concentrations, according to guidelines.

 Results Synopsis

NOEL: 4.94 mg/L

LOEC:  12.0 mg/L                    

Endpoint Effected: Reproduction