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Toxicological information

Specific investigations: other studies

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Administrative data

Endpoint:
cytotoxicity
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Cytotoxic effects of TEGDMA on THP-1 cells in vitro
Author:
Halorli OT et al.
Year:
2009
Bibliographic source:
Med Oral Patol Oral Cir Bucal. 2009 Sep 1; 14 (9): e489-493

Materials and methods

Test guideline
Qualifier:
no guideline required
Principles of method if other than guideline:
Human THP-1 monocytes were exposed to different concentrations of the test substance. After 48 h the numbers of viable, mitotic, apoptotic and necrotic cells were determined microscopically.
GLP compliance:
not specified
Type of method:
in vitro

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Purity not specified, but commercial grade assumed.
Specific details on test material used for the study:
- Name of test material (as cited in study report): Triethylene glycol dimethacrylate, TEGDMA
- Physical state: no data
- Analytical purity: no data
- Impurities (identity and concentrations): no data
- Composition of test material, percentage of components: no data
- Purity test date: no data
- Lot/batch No.: no data
- Expiration date of the lot/batch: no data
- Stability under test conditions: no data
- Storage condition of test material: no data

Results and discussion

Details on results:
The viability of cells incubated with 0.5 mM test substance was reduced to app. 90% (estimated from illustration given in the publication); concentrations of 1 mM and higher were significantly cytotoxic.

Applicant's summary and conclusion

Conclusions:
TREGDMA was significantly cytotoxic to human THP-1 monocytes in concentrations of 1 mM and higher.
Executive summary:

In a cytotoxicity assay human THP-1 monocytes were incubated with TREGDMA in concentrations of 0, 0.5, 1, 2, 4, 8 mM corresponding to 0, 143.16, 286.32, 572.64, 1145.28 and 2290.56 mg/L based on molecular weight (286.32 g/mol) for 48 h. The viability of cells incubated with 0.5 mM TREGDMA was reduced to app. 90% (as estimated from illustration given in the publication); concentrations of 1 mM and higher were significantly cytotoxic.