2,2'-ethylenedioxydiethyl dimethacrylate

Specific investigations: other studies

Administrative data

Endpoint:

cytotoxicity

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Cytotoxic effects of TEGDMA on THP-1 cells in vitro

Author:

Halorli OT et al.

Year:

2009

Bibliographic source:

Med Oral Patol Oral Cir Bucal. 2009 Sep 1; 14 (9): e489-493

Materials and methods

Test guideline

Qualifier:

no guideline required

Principles of method if other than guideline:

Human THP-1 monocytes were exposed to different concentrations of the test substance. After 48 h the numbers of viable, mitotic, apoptotic and necrotic cells were determined microscopically.

GLP compliance:

not specified

Type of method:

in vitro

Test material

Test material information

Constituent 1

Reference substance name:

2,2'-ethylenedioxydiethyl dimethacrylate

EC Number:

203-652-6

EC Name:

2,2'-ethylenedioxydiethyl dimethacrylate

Cas Number:

109-16-0

Molecular formula:

C14H22O6

IUPAC Name:

ethane-1,2-diylbis(oxyethane-2,1-diyl) bis(2-methylacrylate)

Details on test material:

Purity not specified, but commercial grade assumed.

Specific details on test material used for the study:

- Name of test material (as cited in study report): Triethylene glycol dimethacrylate, TEGDMA
- Physical state: no data
- Analytical purity: no data
- Impurities (identity and concentrations): no data
- Composition of test material, percentage of components: no data
- Purity test date: no data
- Lot/batch No.: no data
- Expiration date of the lot/batch: no data
- Stability under test conditions: no data
- Storage condition of test material: no data

Results and discussion

Details on results:

The viability of cells incubated with 0.5 mM test substance was reduced to app. 90% (estimated from illustration given in the publication); concentrations of 1 mM and higher were significantly cytotoxic.

Applicant's summary and conclusion

Conclusions:

TREGDMA was significantly cytotoxic to human THP-1 monocytes in concentrations of 1 mM and higher.

Executive summary:

In a cytotoxicity assay human THP-1 monocytes were incubated with TREGDMA in concentrations of 0, 0.5, 1, 2, 4, 8 mM corresponding to 0, 143.16, 286.32, 572.64, 1145.28 and 2290.56 mg/L based on molecular weight (286.32 g/mol) for 48 h. The viability of cells incubated with 0.5 mM TREGDMA was reduced to app. 90% (as estimated from illustration given in the publication); concentrations of 1 mM and higher were significantly cytotoxic.