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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Specific investigations: other studies

Currently viewing:

Administrative data

Type of information:
experimental study
Adequacy of study:
supporting study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference Type:
Cytotoxic effects of TEGDMA on THP-1 cells in vitro
Halorli OT et al.
Bibliographic source:
Med Oral Patol Oral Cir Bucal. 2009 Sep 1; 14 (9): e489-493

Materials and methods

Test guideline
no guideline required
Principles of method if other than guideline:
Human THP-1 monocytes were exposed to different concentrations of the test substance. After 48 h the numbers of viable, mitotic, apoptotic and necrotic cells were determined microscopically.
GLP compliance:
not specified
Type of method:
in vitro

Test material

Constituent 1
Chemical structure
Reference substance name:
2,2'-ethylenedioxydiethyl dimethacrylate
EC Number:
EC Name:
2,2'-ethylenedioxydiethyl dimethacrylate
Cas Number:
Molecular formula:
ethane-1,2-diylbis(oxyethane-2,1-diyl) bis(2-methylacrylate)
Details on test material:
Purity not specified, but commercial grade assumed.
Specific details on test material used for the study:
- Name of test material (as cited in study report): Triethylene glycol dimethacrylate, TEGDMA
- Physical state: no data
- Analytical purity: no data
- Impurities (identity and concentrations): no data
- Composition of test material, percentage of components: no data
- Purity test date: no data
- Lot/batch No.: no data
- Expiration date of the lot/batch: no data
- Stability under test conditions: no data
- Storage condition of test material: no data

Results and discussion

Details on results:
The viability of cells incubated with 0.5 mM test substance was reduced to app. 90% (estimated from illustration given in the publication); concentrations of 1 mM and higher were significantly cytotoxic.

Applicant's summary and conclusion

TREGDMA was significantly cytotoxic to human THP-1 monocytes in concentrations of 1 mM and higher.
Executive summary:

In a cytotoxicity assay human THP-1 monocytes were incubated with TREGDMA in concentrations of 0, 0.5, 1, 2, 4, 8 mM corresponding to 0, 143.16, 286.32, 572.64, 1145.28 and 2290.56 mg/L based on molecular weight (286.32 g/mol) for 48 h. The viability of cells incubated with 0.5 mM TREGDMA was reduced to app. 90% (as estimated from illustration given in the publication); concentrations of 1 mM and higher were significantly cytotoxic.