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Environmental fate & pathways

Biodegradation in water: screening tests

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Calamari et al (1982) tested the degradation of 8 and 40 mg/l 1,4 -dichlorobenzene by primary sludge in a respirometer test comparable to a MITI(I) test. The degradation of 8 mg/l concentration reached 100%; 40 mg/l probably had a toxic effect and a degradation rate of 30% at day 28. The publication does not state whether the 10 -day-window criterion was respected or not. By plotting the results on a graph it can clearly be seen that more than 60% were degraded 10 days after reaching 10% biodegradation.
Topping (1987) tested 1,4 -dichlorobenzene for rapid biodegradability in a Closed Bottle Test (OECD guideline D). Disappearance of the substance after 28 days (67%) was confirmed by specific HPLC analysis. Thus, the 60% threshold is passed. Due to the lack of intermediate results it is not possible to conclude whether the 10 -day window is respected or not.
As an intermediate metabolite 3,6 -dichlorobrenzcatechine was identified, which however disappeared after 3 hours. (Schraa 1986).
Under anaerobic conditions with sewage sludge from a domestic sewage treatment plant, the substance degrades up to 80% within 32 days. (Kirk 1989).

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

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