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EC number: 937-158-3
CAS number: -
The test acceptance criteria given were
- the amphipods were not held without
sediment for more than 14 days before they were used for testing;
- the mortality in the individual control
replicate did not exceed 40%;
- the entire batch was from the same
- the percentage mortality in the blank was
less than 15 %;
- the oxygen contnentwas greater than 40%;
- the 10 -d LC50 for the fluoranthene was
An acute toxicity test was conducted on the
marine amphipod Corophium volutator. The test substance
concentrations ranged from 126 to 1495 mg/kg sediment dw. The test
sediment was from the same unpolluted site as the amphipods. The
seawater was natural seawater from an unpolluted site at about 80 m
depth in Byfjord, filtered through sand filter at the collection. The
test substance was dissolved in acetone and added to the sediment as
follows: 5 mL acetone containing the test substance was added to 130 g
pre-dried sediment; after the acetone had dried off in a fume cupboard,
the sample was added to 510 g of damp sediment in a plastic bucket, 130
mL seawater was added to the bucket and the bucket was shaken (120 rpm)
for 5 hrs. The spiked sediment was then split into three beakers, 400 mL
of seawater was added to each beaker, and they were aerated overnight
before the test organisms were introduced. The blanks were prepared the
same way (three with and three without acetone). In the test, 4
concentrations were tested in a geometric series: 126, 308, 668 and 1495
mg/kg. Three replicates were used for each concentration and each blank.
Dissolved oxygen was > 40% during the study.
The pH was 8.1. The temperature was 15 +/- 2 °C.
Test substance concentration
Oxygen (% saturation)
126 mg/kg dw
308 mg/kg dw
668 mg/kg dw
1495 mg/kg dw
Reference, 5.57 mg/kg dw
Reference, 10.3 mg/kg dw
Reference, 18.9 mg/kg dw
Reference, 32.0 mg/kg dw
The test organisms (5 -10 mm) were obtained
from the same unpolluted site as the sediment.
No deviation from the protocol was noted. No
analytical monitoring was performed.
Concentration (mg/kg dw)
Total number of specimen per concentration
Number of specimen per vessel
Number of dead
The results mean that the 10 -d LC50
is 503 mg/kg sediment dry weight as shown in the following
(mg/kg sediment dw)
Fluoranthene (reference substance)
The 10d-LC50 and 10d-NOEC for the
marine sediment amphipod Corophium volutator were reported to be
respectively 537 and 171 mg/kg sediment (dw) (geometric mean).
However, as long-term data is not available
for this end-point, the environmental risk is assessed using the
marine amphipod Corophium volutator was exposed to sediment
spiked with Hydrocarbons, C14-C18, n-alkanes, isoalkanes, cyclics, <2%
aromatics for 10 days. Fourteen studies were available and give LC50
values. The geometric mean of LC50 is 537 mg/kg
sediment (dw). In most of the studies, LC10 were calculated
and the geometric mean of the 12 available values is 112.6 mg/kg
sediment (dw). At last, the 7 more recent studies reported NOEC values
and the lowest one was 100 mg/kg (dw).
marine mollusc bivalve Abra alba was exposed to sediment spiked
with Hydrocarbons, C14-C18, n-alkanes, isoalkanes, cyclics, <2%
aromatics for 5 days. Four studies were available and give LC50 values.
The 5-day LC50 value ranged from is 36 +/- 11 to 103 +/- 22
mg test substance / kg 8% (dw) standard sediment suspension.
data is available for long-term sediment toxicity.
accordance with Annex IX column 2, testing does not need to be conducted
as the hazard is adequately assessed by equilibrium partitioning.
Substance is a hydrocarbon UVCB: The hydrocarbon block method is used
for environmental risk assessment (see REACH guidance, R7, app.13-1).
PNECs for hydrocarbon blocks have been derived using aquatic PNECs and
the equilibrium partitioning method (EqP) using representative
structures. Justification for this approach is provided in Redman et
al., 2014. Extension and validation of the target lipid model for
deriving predicted no-effect concentrations for soils and sediments.
ET&C, Vol. 33, No. 12, pp. 2679–2687. See Product Library tab in
“assessment report. PetroRisk information excel” spreadsheet attached to
Section 13 for PNEC values.
aquatic PNECs have been derived using the HC5 statistical extrapolation
method and the target lipid model using representative structures. Given
the large database of organisms included in the target lipid model, an
assessment factor of one has been applied to the HC5.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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