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EC number: 233-071-3
CAS number: 10028-18-9
There were no clinical symptoms of poisoning or mortality among
experimental animals due to the dermal application of nickel sulphate.
No gross changes were noticed in the skin, liver, kidney or testis of
rats painted with nickel sulphate. There was no liver enlargement and
the weight of liver showed no significant difference from those of
controls. The testis did not show any marked atrophy or hypertrophy. The
colour and weight of testes did not differ significantly from those of
The results of microscopic examination on skin, liver and testis of
control animals and those of 40, 60 and 100 mg Ni/kg groups after 15 and
30 days of exposure to nickel demonstrated that a NOAEL of 40 mg Ni/kg
existed for effects on skin testis, and liver of nickel sulfate painted
rats. No abnormality was observed in kidney of nickel sulphate treated
RATED BY AN INDEPENDENT REVIEWER.
SUMMARY DEVELOPED BY AN INDEPENDENT REVIEWER.
Robust Summary for
Mathur et al.(1977):
Appropriate amounts of the test substance were dissolved in normal saline to prepare nominal test concentrations of 40, 60, and 100 mg Ni/kg. Male
albino rats (160 +/- 10 g), obtained from Industrial Toxicology Research Centre (Lucknow, India), were divided into 4 groups of 8. A 4x4-cm patch
of fur was shaved on the lateroabdominal area of each animal. Animals in Group 1 were treated dermally with 40 mg Ni/kg; Group 2 with 60 mg Ni/kg;
Group 3 with 100 mg Ni/kg; and Group 4 (control group) with 0.25 ml untreated saline. The test and control concentrations were administered
at a volume of 0.25 ml. Dermal applications continued daily for the entire 30-day exposure period. The test was conducted under constant
conditions of temperature (25 +/- 2 deg. C) and relative humidity (60-70%). Animals were observed for mortality.
After 15 days of treatment, 4 animals from each group were sacrificed, with the remaining 4 animals sacrificed at the end of the 30-day exposure
period. Gross and histopathological examinations of the skin, liver, kidney, and testis of each animal were conducted.
No mortality or symptoms of poisoning were noted during the exposure
There were no gross changes to the liver, skin, kidneys, or testis of the exposed rats, after either 15- or 30-days of exposure.
The results of microscopic examination of the skin, liver, kidneys, and testis of exposed rats are summarized by treatment group:
Group 1 (40 mg Ni/kg): Day 15 - all tissues normal. Day 30 - slight hyperkeratinization and distortion of the epidermis was noted. No abnormal changes to the liver
Group 2 (60 mg Ni/kg): Day 15 - testis and skin normal; liver hepatocytes swollen with some degeneration. Day 30 - tubular damage, degenerated
sperm, and edematous fluid in testis; slight distortion and hyperkeratinization of the skin; focal necrosis, dilatation of the sinusoids, and vacuolation of the liver.
Group 3 (100 mg Ni/kg): Day 15 - testis normal; distortion of the dermis and epidermis of the skin; liver hepatocytes swollen with some
degeneration. Day 30 - increased tubular degeneration and edema of the testis, necrotic tubules with a few giant cells in one rat, and
distortion of the seminiferous tubules; atrophy or acanthosis of the epidermis, epidermal cells disordered, increasing hyperkeratinization;
focal necrosis, dilatation of the sinusoids, and vacuolation of the liver.
Group 4 (control; 0.25 ml saline): Days 15 and 30 - all tissues normal.
Based on the results presented, there is evidence that the test substance was absorbed through intact skin. Testicular changes were observed at an
application of >= 60 mg Ni/kg, after exposure for 30 days. Minor changes to the liver and skin were observed at 60 and 100 mg Ni/kg, respectively,
following 15 days of continuous exposure. Microscopic changes to the testis, liver, and skin became more severe following 30 days of continuous exposure.
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