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EC number: 227-873-2
CAS number: 6018-92-4
treatment with the negative control the absorbance values were well
within the required acceptability criterion thus showing the quality of
the tissues. The positive control induced a decrease in the relative
absorbance as compared to the negative control to 15.8% thus ensuring
the validity of the test system. After tissue incubation with nickel
hydrogencitrate, the relative absorbance values were increased to
120.7%. This value is well above the threshold for irritancy of 50%.
Therefore, the test item is considered to have no skin irritation
potential. Results see table 1.
Absorbance 570 nm, tissue 1*
Absorbance 570 nm, tissue 2*
Absorbance 570 nm, tissue 3*
Mean absorbance of 3 tissues
Rel. absorbance [% of negative control]**
of two replicate wells after blank correction
relative absorbance [rounded values]: (100 x (absorbance test item)) /
(absorbance negative control)
evaluation of the MTT-reducing capacity of the test item after 1 hour
incubation with MTT reagent did not show blue color.
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