Ammonium N-methyl-N-(1-oxododecyl)glycinate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

no analytics

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A defined amount of the test substance (stock solution = 500 mg/L) was weighed into a measuring flask and the total volume was filled up with dilution water. The test bottles were conditioned about 15 min before the test was started.
- Differential loading: yes
- Controls: dilution water

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: Umwelt-Bundesamt, Berlin, Germany
- Age of daphnia at test initiation: 6 - 24 h
- Feeding during test: no

BREEDING
- Breeding conditions: 20 °C
- Food type: freshwater alga suspension (Scenedesmus subspicatus)
- Frequency: daily

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test temperature:

22 °C

pH:

0 h: 7.5 (control), 7.6 - 7.7 (test solutions)
48 h: 7.5 (control), 7.2 - 7.5 (test solutions)

Dissolved oxygen:

0 h: 5.9 mg/L (control), 5.6 - 6.0 (test solutions)
48 h: 5.7 mg/L (control), 4.3 - 5.7 (test solutions), corresponding to 66% of the air saturation

Nominal and measured concentrations:

nominal: control, 5, 10, 25, 50, 100 and 250 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: beakers
- Fill volume: 10 mL
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: distilled water; Ca/Mg ratio: 4:1, sum = 2.5 mmol/L
- Culture medium different from test medium: no
- measurement of water quality paramaters: The pH and O2 concentration were measured before and after the test

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: no illumination during the test

EFFECT PARAMETERS MEASURED:
- Immobilization: at 24 h and 48 h

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

38.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Mortality of control: 0%

Results with reference substance (positive control):

- Results with reference substance valid? yes
- Relevant effect levels: EC50 (24 h) = 1.2 mg/L

Reported statistics and error estimates:

The statistical evaluation is based on a Probit analysis. The calculation of the probit values was performed with the computer program "Dosis 50". In this program the experimentally determined percentages in the range from 1 - 99% can be used for the calculation of the probit values and for the evaluation of the effective concentrations. For the experimentally determined values 0% and 100% no probit values are defined. For the calculation of the probit values of these percentages the percentages 1% and 99% were used.

The highest tested concentration of test substance producing no immobile daphnia under test conditions after 48 h was 5.0 mg/L.

The lowest tested concentration of test substance producing 100% immobile daphnia under test conditions after 48 h was 250 mg/L.

Table 1. Study results.

Concentration of test substance [mg/L]	Immobilization [%]		pH-value		O2 [mg/L]
	24 h	48 h	Before test	After test	Before test	After test
250	100	100	7.6	7.3	5.6	4.3
100	60	95	7.7	7.2	5.7	4.4
50	25	45	7.6	7.3	5.7	4.7
25	10	35	7.6	7.5	5.5	5.4
10	10	10	7.7	7.4	6.0	5.7
5.0	0	0	7.6	7.5	6.0	5.7
control	0	0	7.5	7.5	5.9	5.7

 

Validity criteria fulfilled:

yes

Description of key information

EC50 (48 h) = 38.4 mg/L (nominal, Daphnia magna, OECD 202)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

38.4 mg/L

Additional information

One GLP-study according to OECD 202 investigating the short-term toxicity of Ammonium N-methyl-N-(1-oxododecyl)glycinate (CAS 68003-46-3) to aquatic invertebrates is available. The test organism Daphnia magna was exposed for 48 h to six nominal test concentrations (5, 10, 25, 50, 100 and 250 mg/L). No analytical monitoring of test concentrations was performed. Mortality was recorded every 24 h and was compared to the negative control. After 48 h immobilisation was observed at concentrations ≥ 10 mg/L (nominal). The derived nominal EC50 (48 h) value was 38.4 mg/L.