Ammonium N-methyl-N-(1-oxododecyl)glycinate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A defined amount of the test substance (stock solution = 2000 mg/L) was weighed into a measuring flask and the total volume was filled up with dilution water. The test bottles were conditioned about 15 min before the test was started.
- Differential loading: yes
- Controls: dilution water

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrafish
- Length at study initiation: 2.0 cm (average length)

ACCLIMATION
- Acclimation period: at least 14 d
- Acclimation conditions: same as test
- Feeding frequency during acclimation: daily

FEEDING DURING TEST
- Frequency: no feeding

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

0 h: 18.7 °C (control), 18.7 - 18.8 °C (test solutions)
96 h: 18.8 °C (control), 18.5 - 18.8 °C (test solutions)

pH:

0 h: 7.5 (control), 7.6 - 7.7 (test solutions)
96 h: 7.5 (control), 7.7 - 7.9 (test solutions)

Dissolved oxygen:

0 h: 7.1 mg O2/L (control), 6.6 - 8.1 mg O2/L (test solutions)
96 h: 6.3 mg O2/L (control), 6.8 - 7.9 mg O2/L (test solutions)

Nominal and measured concentrations:

nominal: control, 10, 25, 50, 100 and 200 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: beakers
- Fill volume: 7 L
- Aeration: yes, constant
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: chlorine-free drinking water; hardness: 37.4 mg/L Ca-content
- Metals: Copper content < 0.01 mg/L
- Chlorine: none
- Culture medium different from test medium: no
- Intervals of water quality measurement: The pH, O2 concentration and temperature were measured at 0, 24, 48, 72 and 96 h.

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 12 h light/dark cycle
- Light intensity: indirect day-similar light

EFFECT PARAMETERS MEASURED:
The fish were inspected after 24, 48, 72 and 96 h.

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 - 2.5
- Range finding study: no

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

95.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Mortality of control: 0%

Reported statistics and error estimates:

The statistical evaluation is based on a Probit analysis. The calculation of the probit values with the date after 24 to 96 h was performed with the computer program "Dosis 50". For the experimental values of 0% and 100% no probit values are defined. For the calculation of the probit values of these percentages the percentages 1% and 99% were used.

Sublethal observations / clinical signs:

The highest tested concentration of test substance producing no mortality under test conditions after 96 h was 50 mg/L.

The lowest tested concentration of test substance producing 100% mortality under test conditions after 96 h was 200 mg/L.

Table 1. Mortality in 24 h intervals.

Concentration of test substance [mg/L]	Mortality [%] After 24 h	Mortality [%] After 48 h	Mortality [%] After 72 h	Mortality [%] After 96 h
200	100	100	100	100
100	14	57	57	57
50	0	0	0	0
25	0	0	0	0
10	0	0	0	0
control	0	0	0	0

 

Validity criteria fulfilled:

yes

Description of key information

LC50 (96 h) = 95.8 mg/L (nominal, OECD 203, Danio rerio)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

95.8 mg/L

Additional information

One GLP-study according to OECD 203 investigating the short-term toxicity of Ammonium N-methyl-N-(1-oxododecyl)glycinate (CAS 68003-46-3) to freshwater fish is available. The test organism Danio rerio was exposed for 96 h to five nominal test concentrations (10, 25, 50, 100 and 200 mg/L). No analytical monitoring of test concentrations was performed. Mortality was recorded daily and was compared to the negative control. After 96 h mortality was observed at concentrations ≥ 100 mg/L (nominal). The derived nominal LC50 value after 96 h was 95.8 mg/L (nominal).