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EC number: 222-048-3
CAS number: 3327-22-8
1: Effect of test material on growth and survival of D. magna
(average per 10 replicates unless otherwise stated) during the 21
Treatment (mg a.i./L) nominal concentration
Mortality of parents (dead or immobile)
Mean no. of offspring
No. of juveniles per parent alive (mean ± SD)
Day of first appearance of juveniles
Mean length (mm)
Mean weight (mg)
No. Dead (per 10 replicates)
% (per 10 replicates)
108 ± 5.04
0 ± 0
130 ± 13
107 ± 11.3
106 ± 8.54
denotes when an endpoint is not applicable, e.g. the parent daphnia
died before the end of the test and it was not possible to measure
length and weight.
2: Parental daphnia immobilisation results.
Result (mg a.i./L, nominal)
3: Nominal and measured concentrations (ranges) during exposure.
Measured concentration (mg a.i./L) results
EPTAC as CHPTAC
range (min. - max.)
Sum range (min. - max.)
Old media (48 h)
Sum range (min.-max.)
calculable because concentrations measured for EPTAC were <LOQ.
were only taken where living test organisms were present so for
higher treatment value are not representative of the whole 21 day
A 21 day NOEC value of 0.51 mg a.i./L has
been determined for the effects of the test substance on reproduction of
the test organism D. magna. The test was conducted according to the OECD
guideline 211 and GLP.
The measured concentration are not within 80
% of the nominal concentrations. Given Effect concentrations are based
on nominal values, following the approach in the EU Risk Assessment
Report on CHPTAC (2008).
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