(3-chloro-2-hydroxypropyl)trimethylammonium chloride

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

unsuitable test system

Principles of method if other than guideline:

Deutsche Einheitsverfahren L3 (TTC-Test). The test is based on the reduction of 2,3,5-triphenyltetrazoliumchloride (TTC).

GLP compliance:

no

Analytical monitoring:

not specified

Test organisms (species):

activated sludge

Details on test conditions:

Type: aquatic

Dose descriptor:

EC0

Effect conc.:

ca. 5 115 mg/L

Highest test concentration (9.3 g/l QUAB) caused no effects on the Dehydogenaseactivity of the sludge. As the QUAB contains ca. 55 % 3-Chloro-2-hydroxypropyl)trimethylammoniumchloride, the more correct test concentration would be 5.115 g/l.

Conclusions:

This test does not fulfil the requirements for the micro-organisms toxicity test, since it only measures the inhibition of dehydrogenase activity of the sludge. Many microorganisms can suffer or even be dead although the enzyme activity can be detected.

Reference 2

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

unsuitable test system

Remarks:

No dose-response has been presented in the report regarding CHPTAC concentration in the waste water, and therefore the study has not been considered relevant for assessment.

Principles of method if other than guideline:

Method: other: fill and draw test

GLP compliance:

not specified

Analytical monitoring:

not specified

Test organisms (species):

other: Bacterial sludge from industrial sewage treatment plant, which had been grown using wastewater from an unit, which did not use quab 188

Details on test conditions:

Type: aquatic

Dose descriptor:

EC0

Effect conc.:

765 mg/L

Conclusions:

It was stated that the test substance addition up to 1500 mg/l (765 mg/l since the concentration was 51 %) was not toxic or inhibitory to biological treatment of the industrial wastewater. According to the report the bacteria had become acclimated to test substance and were at the end able to partially remove it. Aqueous solution of CHPTAC was 51 % and therefore result was corrected as 100 % CHPTAC.

Reference 3

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Principles of method if other than guideline:

Method: other: agar-agar plate method; no further data

GLP compliance:

not specified

Analytical monitoring:

not specified

Test organisms (species):

Aspergillus niger

Details on test conditions:

Type: other: agar-agar

Dose descriptor:

other: EC

Effect conc.:

> 3 000 mg/L

Conclusions:

EC > 3000 mg/L (Aspergillus niger)

Reference 4

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Principles of method if other than guideline:

Method: other: agar-agar plate method; no further data

GLP compliance:

not specified

Analytical monitoring:

not specified

Test organisms (species):

Escherichia coli

Details on test conditions:

Type: other: agar-agar

Dose descriptor:

other: EC

Effect conc.:

> 3 000 mg/L

Conclusions:

EC > 3000 mg/L (Escherichia coli)

Reference 5

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Documentation insufficient for assessment

Principles of method if other than guideline:

Method: other: according to Bringmann, Kühn (1977)

GLP compliance:

not specified

Analytical monitoring:

not specified

Test organisms (species):

Pseudomonas putida

Details on test conditions:

Type: aquatic

Duration:

16 h

Dose descriptor:

other: EC

Effect conc.:

> 20 000 mg/L

Conclusions:

EC > 2000 mg/L (Pseudomonas putida)

Reference 6

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Principles of method if other than guideline:

Method: agar plate method; no further data

GLP compliance:

not specified

Analytical monitoring:

not specified

Test organisms (species):

Staphylococcus aureus

Details on test conditions:

Type: other: agar agar

Dose descriptor:

other: EC

Effect conc.:

> 3 000 mg/L

Conclusions:

EC > 3000 mg/L (Staphylococcus aureus)

Reference 7

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

activated sludge of a predominantly domestic sewage

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Details on test conditions:

Type: aquatic

Key result

Duration:

3 h

Dose descriptor:

EC10

Effect conc.:

1 032 mg/L

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

> 2 000 mg/L

Remarks on result:

other: IUCLID4 note: "m" (measured/nominal)

Duration:

3 h

Dose descriptor:

other: EC20

Effect conc.:

> 2 000 mg/L

Remarks on result:

other: IUCLID4 note: "m" (measured/nominal)

No EC50-value could be found with the highest test concentration of 2000 mg/l. In the test report EC20-, EC50 - and EC80-values higher than 2000 mg/l have been reported, but no EC10-value has been estimated. Based on graphical estimation EC10-value of 1225 mg/l can be extrapolated.
There were no estimations of goodness of fit parameters available in graphs (standard deviation, R2 etc.)

In the addendum to the test report EC10-value has been estimated to be 1032 mg/l.

Validity criteria fulfilled:

yes

Conclusions:

In a good quality study conducted according to OECD Test Guideline 209 and in compliance with GLP, the EC50 for respiration inhibition of activated sludge was determined to be >2000 mg/l. This result is expressed as active ingredient.

Reference 8

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

GLP compliance:

yes

Test organisms (species):

Pseudomonas putida

Details on test conditions:

Type: aquatic

Duration:

19 h

Dose descriptor:

EC50

Effect conc.:

58 g/L

Remarks on result:

other: calculated

At the highest concentration tested (32 g/l) a growth inhibition of 10% was found. The toxicity threshold was, therefore 32 g/l. According to the test report from these data an EC50 of 58 g/l with respect to the growth rate was extrapolated.

Conclusions:

Pseudomonas putida
19 h EC10 = 32 g/L (toxicity threshold)
19 h EC50 = 58 g/L (extrapolated value

Reference 9

Endpoint:

toxicity to microorganisms

Type of information:

read-across based on grouping of substances (category approach)

Adequacy of study:

supporting study

Justification for type of information:

Read-across information from the Diol is included in the present dossier as it is the main degradation product taken into account in the EU Risk Assessment Report on CHPTAC (2008).

Duration:

19 h

Dose descriptor:

EC50

Effect conc.:

58 g/L

Remarks on result:

other: calculated

At the highest concentration tested (32 g/l) a growth inhibition of 10% was found. The toxicity threshold was, therefore 32 g/l. According to the test report from these data an EC50 of 58 g/l with respect to the growth rate was extrapolated.

Conclusions:

Pseudomonas putida
19 h EC10 = 32 g/L (toxicity threshold)
19 h EC50 = 58 g/L (extrapolated value

This information is used in a read-across approach in the assessment of the target substance.

Description of key information

The key study to assess toxicity towards microorganisms was conducted according to OECD 209 and GLP. The EC10 was determined with 1032 mg/L.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:

1 032 mg/L

Additional information

Besides the key study several information sources are reported indictating that CHPTAC is not toxic to microorganisms. Read-across information from the Diol is included in the present dossier as it is the main degradation product taken into account in the EU Risk Assessment Report on CHPTAC (2008). An EC10 of 32g/L was determined for the diol.