Registration Dossier
Registration Dossier
Diss Factsheets
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EC number: 222-048-3 | CAS number: 3327-22-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- unsuitable test system
- Principles of method if other than guideline:
- Deutsche Einheitsverfahren L3 (TTC-Test). The test is based on the reduction of 2,3,5-triphenyltetrazoliumchloride (TTC).
- GLP compliance:
- no
- Analytical monitoring:
- not specified
- Test organisms (species):
- activated sludge
- Details on test conditions:
- Type: aquatic
- Dose descriptor:
- EC0
- Effect conc.:
- ca. 5 115 mg/L
- Conclusions:
- This test does not fulfil the requirements for the micro-organisms toxicity test, since it only measures the inhibition of dehydrogenase activity of the sludge. Many microorganisms can suffer or even be dead although the enzyme activity can be detected.
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- unsuitable test system
- Remarks:
- No dose-response has been presented in the report regarding CHPTAC concentration in the waste water, and therefore the study has not been considered relevant for assessment.
- Principles of method if other than guideline:
- Method: other: fill and draw test
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Test organisms (species):
- other: Bacterial sludge from industrial sewage treatment plant, which had been grown using wastewater from an unit, which did not use quab 188
- Details on test conditions:
- Type: aquatic
- Dose descriptor:
- EC0
- Effect conc.:
- 765 mg/L
- Conclusions:
- It was stated that the test substance addition up to 1500 mg/l (765 mg/l since the concentration was 51 %) was not toxic or inhibitory to biological treatment of the industrial wastewater. According to the report the bacteria had become acclimated to test substance and were at the end able to partially remove it. Aqueous solution of CHPTAC was 51 % and therefore result was corrected as 100 % CHPTAC.
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Principles of method if other than guideline:
- Method: other: agar-agar plate method; no further data
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Test organisms (species):
- Aspergillus niger
- Details on test conditions:
- Type: other: agar-agar
- Dose descriptor:
- other: EC
- Effect conc.:
- > 3 000 mg/L
- Conclusions:
- EC > 3000 mg/L (Aspergillus niger)
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Principles of method if other than guideline:
- Method: other: agar-agar plate method; no further data
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Test organisms (species):
- Escherichia coli
- Details on test conditions:
- Type: other: agar-agar
- Dose descriptor:
- other: EC
- Effect conc.:
- > 3 000 mg/L
- Conclusions:
- EC > 3000 mg/L (Escherichia coli)
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Documentation insufficient for assessment
- Principles of method if other than guideline:
- Method: other: according to Bringmann, Kühn (1977)
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Test organisms (species):
- Pseudomonas putida
- Details on test conditions:
- Type: aquatic
- Duration:
- 16 h
- Dose descriptor:
- other: EC
- Effect conc.:
- > 20 000 mg/L
- Conclusions:
- EC > 2000 mg/L (Pseudomonas putida)
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Principles of method if other than guideline:
- Method: agar plate method; no further data
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Test organisms (species):
- Staphylococcus aureus
- Details on test conditions:
- Type: other: agar agar
- Dose descriptor:
- other: EC
- Effect conc.:
- > 3 000 mg/L
- Conclusions:
- EC > 3000 mg/L (Staphylococcus aureus)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Details on test conditions:
- Type: aquatic
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1 032 mg/L
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 2 000 mg/L
- Remarks on result:
- other: IUCLID4 note: "m" (measured/nominal)
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 2 000 mg/L
- Remarks on result:
- other: IUCLID4 note: "m" (measured/nominal)
- Validity criteria fulfilled:
- yes
- Conclusions:
- In a good quality study conducted according to OECD Test Guideline 209 and in compliance with GLP, the EC50 for respiration inhibition of activated sludge was determined to be >2000 mg/l. This result is expressed as active ingredient.
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- GLP compliance:
- yes
- Test organisms (species):
- Pseudomonas putida
- Details on test conditions:
- Type: aquatic
- Duration:
- 19 h
- Dose descriptor:
- EC50
- Effect conc.:
- 58 g/L
- Remarks on result:
- other: calculated
- Conclusions:
- Pseudomonas putida
19 h EC10 = 32 g/L (toxicity threshold)
19 h EC50 = 58 g/L (extrapolated value - Endpoint:
- toxicity to microorganisms
- Type of information:
- read-across based on grouping of substances (category approach)
- Adequacy of study:
- supporting study
- Justification for type of information:
- Read-across information from the Diol is included in the present dossier as it is the main degradation product taken into account in the EU Risk Assessment Report on CHPTAC (2008).
- Duration:
- 19 h
- Dose descriptor:
- EC50
- Effect conc.:
- 58 g/L
- Remarks on result:
- other: calculated
- Conclusions:
- Pseudomonas putida
19 h EC10 = 32 g/L (toxicity threshold)
19 h EC50 = 58 g/L (extrapolated value
This information is used in a read-across approach in the assessment of the target substance.
Referenceopen allclose all
Highest test concentration (9.3 g/l QUAB) caused no effects on the Dehydogenaseactivity of the sludge. As the QUAB contains ca. 55 % 3-Chloro-2-hydroxypropyl)trimethylammoniumchloride, the more correct test concentration would be 5.115 g/l.
No EC50-value could be found with the highest test
concentration of 2000 mg/l. In the test report EC20-, EC50 -
and EC80-values higher than 2000 mg/l have been reported,
but no EC10-value has been estimated. Based on graphical
estimation EC10-value of 1225 mg/l can be extrapolated.
There were no estimations of goodness of fit parameters
available in graphs (standard deviation, R2 etc.)
In the addendum to the test report EC10-value has been
estimated to be 1032 mg/l.
At the highest concentration tested (32 g/l) a growth inhibition of 10% was found. The toxicity threshold was, therefore 32 g/l. According to the test report from these data an EC50 of 58 g/l with respect to the growth rate was extrapolated.
At the highest concentration tested (32 g/l) a growth inhibition of 10% was found. The toxicity threshold was, therefore 32 g/l. According to the test report from these data an EC50 of 58 g/l with respect to the growth rate was extrapolated.
Description of key information
The key study to assess toxicity towards microorganisms was conducted according to OECD 209 and GLP. The EC10 was determined with 1032 mg/L.
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 032 mg/L
Additional information
Besides the key study several information sources are reported indictating that CHPTAC is not toxic to microorganisms. Read-across information from the Diol is included in the present dossier as it is the main degradation product taken into account in the EU Risk Assessment Report on CHPTAC (2008). An EC10 of 32g/L was determined for the diol.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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