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Toxicological information

Additional toxicological data

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Administrative data

Endpoint:
additional toxicological information
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
Detailed account of vapour test atmosphere generation methodology used for toxicity studies of gasoline and gasoline/oxygenate mixtures (including gasoline-MTBE), with extensive chemical analysis). Study in itself reliable, but of questionable relevance to toxicity assessment of MTBE.

Data source

Reference
Reference Type:
publication
Title:
Health assessment of gasoline and fuel oxygenate vapors: Generation and characterization of test materials
Author:
Henley M et al
Year:
2014
Bibliographic source:
Regulatory Toxicology and Pharmacology 70 (2014) S13–S17

Materials and methods

Type of study / information:
Description of test vapours produced from baseline gasoline and gasoline with added oxygenate (including gasoline + MTBE): methods used to generate vapours, collect vapour condensates and regenerate vapours used in various toxicological studies. Includes analytical characterisation of the vapour condensates.
Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
Development of methods for vapour generation compatible with specific EPA requirements (211b rule at CFR 79.57(f)(2)) but modified from that to permit safe and reproducible conditiond on testing laboratories
GLP compliance:
no
Remarks:
Not generally applicable, but GLP-compliant analyses included

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Type:
Constituent
Test material form:
other: Vapours collected as condensates
Details on test material:
BG = Reformulated gasoline summer baseline fuel meeting CAA section 211(k)(10)(B)(i) specification (40 CFR 79.55)
BGVC = vapour condensate collected from BG
BG+MTBE = BG with MTBE added to create 2.7 wt% O2 content (corresponding to 14.9 vol% MTBE)
G/MTBE = vapour condensate from BG+MTBE

Results and discussion

Any other information on results incl. tables

Collected vapour condensates represented 16 vol% (BGVC) or 17 vol% (G/MTBE) of the initial liquids (BG or BG+MTBE respectively). Analysis of equilibrium vapour in headspace above BG+MTBE heated to 130F/54C in 20 ml vials indicated an MTBE vapour content of 19.3% (GC-FID, Chevron SE-30 analysis): using this as a target, MTBE vapour content of 21.3 vol% was measured during the large-scale generation of G+MTBE vapour for test article (G/MTBE vapour condensate) collection, using a slightly different GC-FID method under GLP.

Similarity of baseline gasoline vapour condensate to vapour above heated gasoline.

Carbon number component

Liquid gasoline

Equilibrium vapour above gasoline at 130F/54C (vol%)

BGVC condensate (vol%)

C3

0

0

0.1

C4

4.7

27.1

20.6

C5

16.3

40.9

46.4

C6

18.5

18.8

21.6

C7

19.1

8.0

9.0

C8

20.2

4.0

2.2

C9

10.6

0.8

0.2

C10

6.0

0.2

0

C11

2.8

0

0

C12

1.8

00.1

0

GC-FID analysis using proprietary (Chevron SE-30) analysis

Applicant's summary and conclusion

Conclusions:
A large-scale procedure for generation of vapours and collection of vapour condensates from gasoline alone and gasoline with added oxygenates (including MTBE) was developed and described. Composition of the vapour condensate so collected from gasoline was shown to be similar to that of equilibrium vapour above heated liquid gasoline.