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Ecotoxicological information

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Description of key information

Short Term Toxicity to Fish

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test was conducted in static conditions for the period of 96 hours. The test concentration used for the exposure are 2.24 mg/L, 3.36 mg/L, 5.05 mg/L, 7.58 mg/L & 11.37 mg/L.Plastic aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. After 96 hours of exposure to test item to various nominal test concentrations, LC0 was determined to be 11.37 mg/l .

As the chemical is readily biodegradable, it can be consider that the test chemical is non hazardous to aquatic enviornmrnt and cannot be classified as per CLP classsifiaction criteria.

Long Term Toxicity to Fish

Based on the prediction done using ECOSAR version 1.11, the long term toxicity on fish was predicted for test substance . On the basis of effects observed in a flow through freshwater system, the NOEC value for the test substance is estimated to be 0.568 mg/l for aquatic vertebrate for 28 days of exposure,

Based on the obtained value the test chemical can be classified into aquatic chronic 3.

Short term toxicity to aquatic invertebrates

Aim of this study was to assess the short-term toxicity of test chemical to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs.

The test chemical of various concentrations i.e., 3.0, 6.0, 12.0, 25.0, and 50.0 mg/L were used in the study. After the exposure of chemical for 48 hrs, Effect concentration EC50 was determined as 16.8 mg/L.

As the chemical was found to be readily biodegradable, It can be considered to be Not Classified chemical as per the CLP classification criteria.

Long term toxicity to aquatic invertebrates

The effect of the test chemical on the daphnia magna was studied for the period of 21 days. baseed on the ECOSAR prediction tool version 1.11 the NOEC value of the test chemical was predicted as 2.021 mg/L. Thus on the basis of NOEC value, chemical consider to be not classified.

Toxicity to aquatic algae and cyanobacteria

Aim of this study was to evaluate the inhibition of growth on fresh water algae species Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201. The stock solution 10g/L was prepared by dissolving white powder in acetone. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Along With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs. The median effective concentration (EC50) for the test substance, in algae was determined to be 3.4 mg/L on the basis of growth rate inhibition effects in a 72 hours study. Based on CLP classification criteria the test chemical is non toxic to aquatic enviornment and cannot be classsified, as chemical is readily biodegradable.

Toxicity to microorganisms

study provide a means topredict the ‘minimum’ toxicity that a phenol willexert in the Microtox test.Toxicity data noted as ‘pT’ are listed as the negative logarithm of the millimolar concentration required to elicit a 50% reduction in light emission in the stated time (i.e. 5-, 15, or 30-min) and pT 30 was predicted to be 1.09.

Additional information

Short Term Toxicity to Fish

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test was conducted in static conditions for the period of 96 hours. The test concentration used for the exposure are 2.24 mg/L, 3.36 mg/L, 5.05 mg/L, 7.58 mg/L & 11.37 mg/L.Plastic aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. After 96 hours of exposure to test item to various nominal test concentrations, LC0 was determined to be 11.37 mg/l .

Long Term Toxicity to Fish

Based on the prediction done using ECOSAR version 1.11, the long term toxicity on fish was predicted for test substance . On the basis of effects observed in a flow through freshwater system, the NOEC value for the test substance is estimated to be 0.568 mg/l for aquatic vertebrate for 28 days of exposure. On the basis of NOEC value, chemical consider to be classified in aquatic chronic category 3.

Short term toxicity to aquatic invertebrates

Aim of this study was to assess the short-term toxicity of test chemical to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs. The test chemical of various concentrations i.e., 3.0, 6.0, 12.0, 25.0, and 50.0 mg/L were used in the study. After the exposure of chemical for 48 hrs, Effect concentration EC50 was determined as 16.8 mg/L.

Long term toxicity to aquatic invertebrates

The effect of the test chemical on the daphnia magna was studied for the period of 21 days. baseed on the ECOSAR prediction tool version 1.11 the NOEC value of the test chemical was predicted as 2.021 mg/L.

Toxicity to aquatic algae and cyanobacteria

Aim of this study was to evaluate the inhibition of growth on fresh water algae species Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201. The stock solution 10g/L was prepared by dissolving white powder in acetone. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Along With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs. The median effective concentration (EC50) for the test substance, in algae was determined to be 3.4 mg/L on the basis of growth rate inhibition effects in a 72 hours study.

Toxicity to microorganisms

WoE is prepared based on the structural and functional similar RA

First study provide a means topredict the ‘minimum’ toxicity that a phenol willexert in the Microtox test.Toxicity data noted as ‘pT’ are listed as the negative logarithm of the millimolar concentration required to elicit a 50% reduction in light emission in the stated time (i.e. 5-, 15, or 30-min) and pT 30 was predicted to be 1.09.

First study was supported by second from peer reviewed journal, provide a means topredict the ‘minimum’ toxicity that a phenol willexert in the Microtox test.Toxicity data noted as ‘pT’ are listed as the negative logarithm of the millimolar concentration required to elicit a 50% reduction in light emission in the stated time (i.e. 5-, 15, or 30-min) and pT 30, pT15 and pT5 was predicted to be 1.52, 1.59 and 1.66 respectively.

Based on CLP classification criteria the test chemical is toxic to aquatic enviornment and can be classsified as aquatic chronic category 3.