Registration Dossier

Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1996-05-14 to 1996-08-27
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: DIN Guideline under GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996
Report Date:
1996

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: DIN 38412, Teil 8 März 1991

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Monochloracetone
- Physical state: liquid
- Analytical purity: 96.18 %
- Impurities (identity and concentrations): 1,3-Dichloracetone (0.5%); 4-Methylpent-3-en-2-on (1%); 1,1-Dichloracetone (3%)
- Lot/batch No.: 603015
- Expiration date of the lot/batch: March 12, 1997
- Stability under test conditions: room temperature, dark
- Storage condition of test material: moderat stable

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Pseudomonas putida

Study design

Test type:
static
Water media type:
not specified
Limit test:
no
Total exposure duration:
16 h

Test conditions

Test temperature:
21+/-1°C
pH:
test begin 7.2
Nominal and measured concentrations:
nominal 10, 5, 1, 0.5, 0.1, 0.05 mg/L
Reference substance (positive control):
yes
Remarks:
3,5-dichlorphenol

Results and discussion

Effect concentrations
Duration:
16 h
Dose descriptor:
IC50
Effect conc.:
2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: degree of turbidity
Results with reference substance (positive control):
IC50= 9.8 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Monochloroacetone affected the growth of Pseudomonas putida. The IC50 was determinded to be 2.0 mg/L
Executive summary:

The inhibitory effect of monochloroacetone on the growth rate of Pseudomonas putida was examined in a DIN 38412 compliant test. After 16 hours the growth rate was determimed by measuring the degree of turbidity. The IC 50 was graphically estimated to be 2.0 mg/L.