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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
toxicity to microorganisms
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1996-05-14 to 1996-08-27
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: DIN Guideline under GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996
Report date:
1996

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: DIN 38412, Teil 8 März 1991

Test material

Constituent 1
Chemical structure
Reference substance name:
Chloroacetone
EC Number:
201-161-1
EC Name:
Chloroacetone
Cas Number:
78-95-5
Molecular formula:
C3H5ClO
IUPAC Name:
1-chloropropan-2-one
Details on test material:
- Name of test material (as cited in study report): Monochloracetone
- Physical state: liquid
- Analytical purity: 96.18 %
- Impurities (identity and concentrations): 1,3-Dichloracetone (0.5%); 4-Methylpent-3-en-2-on (1%); 1,1-Dichloracetone (3%)
- Lot/batch No.: 603015
- Expiration date of the lot/batch: March 12, 1997
- Stability under test conditions: room temperature, dark
- Storage condition of test material: moderat stable

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Pseudomonas putida

Study design

Test type:
static
Water media type:
not specified
Limit test:
no
Total exposure duration:
16 h

Test conditions

Test temperature:
21+/-1°C
pH:
test begin 7.2
Nominal and measured concentrations:
nominal 10, 5, 1, 0.5, 0.1, 0.05 mg/L
Reference substance (positive control):
yes
Remarks:
3,5-dichlorphenol

Results and discussion

Effect concentrations
Duration:
16 h
Dose descriptor:
IC50
Effect conc.:
2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: degree of turbidity
Results with reference substance (positive control):
IC50= 9.8 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Monochloroacetone affected the growth of Pseudomonas putida. The IC50 was determinded to be 2.0 mg/L
Executive summary:

The inhibitory effect of monochloroacetone on the growth rate of Pseudomonas putida was examined in a DIN 38412 compliant test. After 16 hours the growth rate was determimed by measuring the degree of turbidity. The IC 50 was graphically estimated to be 2.0 mg/L.