Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1996-05-14 to 1996-08-23
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: According to DIN Guideline under GLP, dose range selection not comprehensive.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996
Report Date:
1996

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: DIN 38412, Teil 15 Juni 1982
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Monochloracetone
- Physical state: liquid
- Analytical purity: 96.18 %
- Impurities (identity and concentrations): 1,3-Dichloracetone (0.5%); 4-Methylpent-3-en-2-on (1%); 1,1-Dichloracetone (3%)
- Lot/batch No.: 603015
- Expiration date of the lot/batch: March 12, 1997
- Stability under test conditions: room temperature, dark
- Storage condition of test material: moderat stable

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes
Details on test solutions:
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Methanol
- Stock solution: 200mg test substance + 5000 mg methanol in 1 L test water
- Renewal: After 24 hours

Test organisms

Test organisms (species):
Leuciscus idus
Details on test organisms:
TEST ORGANISM
- Common name:
- Strain:
- Source: Gunter Rosenhainer, 06766 Thalheim
- Length at study initiation (length definition, mean, range and SD): 6.5 cm
- Weight at study initiation (mean and range, SD): 2.4 g
- Feeding during test
- Food type: frozen larve mosquitos
- Frequency: daily until 48 h before test start


Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
-

Test conditions

Hardness:
c(Ca,Mg)= 2.5 mmol
Test temperature:
19.5 -21.0 °C
pH:
7.8-7.9
Dissolved oxygen:
9.0 at 0h
Salinity:
-
Nominal and measured concentrations:
nominal 0, 0.1, 0.2, 0.4, 0.8, 1.6
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
0.6 mg/L
Nominal / measured:
nominal
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
LC0
Effect conc.:
0.2 mg/L
Nominal / measured:
nominal
Basis for effect:
mortality

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Monochloracetone was very toxic to fish under the reported conditions of the test.
Executive summary:

The acute toxicity of monochloroacetone to fish (Leuciscus idus) was tested in a semi-static test according to DIN 38412, Teil 15 Juni 1982. The fish were exposed to the substance in a nominal concentration range from 0.1 mg/L to 1.6 mg/L for 48 hours. The measured concentrations did not drop below 80% of the nominal values. No mortalities occured during the test in the control. The 48 -hour LC50 was 0.6 mg/L.