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Toxicological information

Dermal absorption

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Administrative data

Endpoint:
dermal absorption in vitro / ex vivo
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Well documented, acceptable publication

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Unnamed
Year:
1995
Reference Type:
study report
Title:
Unnamed
Year:
1993
Report Date:
1993

Materials and methods

Principles of method if other than guideline:
Flowthrough in vitro skin penetration chamber method according to Frantz et el. (1990).
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Acrylic acid
- Analytical purity: > 98 % (unlabeled AA)
- Supplier: Union Carbide Corporation

- Radiochemical purity (if radiolabelling): >= 98.6 %
- Specific activity (if radiolabelling): 0.14 - 0.4 mCi/mmol
- Locations of the label (if radiolabelling): [1-14C]AA
- Supplier: Sigma Chemical Co. (St. Louis, Mo.)
Radiolabelling:
yes

Test animals

Species:
rat
Strain:
Fischer 344
Sex:
male
Details on test animals and environmental conditions:
TEST ANIMALS
- Source: Charles River Breeding Laboratories (Kingston, NY)
- Substrain: F344/NHsd
- Age at study initiation: approx. 7 wk old
- Weight at study initiation: 210 g
- Diet (ad libitum): Agway Prolab Diet Rat, Agway Inc., Syracuse, NY
- Water (ad libitum)

Administration / exposure

Vehicle:
acetone
Duration of exposure:
6 hrs
Control animals:
no
Details on in vitro test system (if applicable):
SKIN PREPARATION
- Source of skin: dorsal skin from male rats (hair clipped)


PRINCIPLES OF ASSAY
- Diffusion cell: flow-through diffusion cell (Frantz cell)
- Flow-through system: yes
- Test temperature: 32 °C
- Occlusion: no

Results and discussion

Absorption in different matrices:
Over a 6-h period, 23.9 ± 5.4% (mean ± SD, n= 3) of the dose was absorbed into the effluent or was found in the skin. At least 60% of the dose evaporated, and total recovery of the applied dose was about 85%.
Total recovery:
85 %

Any other information on results incl. tables

The results from the in vitro experiment supported those of the in vivo study (see 7.1.1).

Applicant's summary and conclusion