Registration Dossier
Registration Dossier
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EC number: 231-209-7 | CAS number: 7446-81-3
Toxicity to soil macroorganisms except arthropods
Administrative data
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 20 Sep 1995 - 04 Oct 1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.8 (Toxicity for Earthworms: Artificial Soil Test)
- Deviations:
- yes
- Remarks:
- average live weights of worms were not determined at test end
- GLP compliance:
- yes
Test material
- Reference substance name:
- Acrylic acid
- EC Number:
- 201-177-9
- EC Name:
- Acrylic acid
- Cas Number:
- 79-10-7
- Molecular formula:
- C3H4O2
- IUPAC Name:
- acrylic acid
- Details on test material:
- - Name of test material (as cited in study report): Acrylic acid
- Substance type: pure substance
- Physical state: liquid
- Analytical purity: 99.78 % (mass, GC)
- Impurities (identity and concentrations): stabilised with 200 ppm hydrochinone monomethylether
- Stability under test conditions: stable
- ID No. of the testing laboratory: 0637/81718
- Sample No.: 1529/950420
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test substrate
- Vehicle:
- yes
- Details on preparation and application of test substrate:
- - Chemical name of vehicle: water
- Concentration of vehicle in test medium (stock solution): 2.67 g/L
Test organisms
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- TEST ORGANISM
- Common name: Earthworm
- Source: Eisenia GmbH & Co., Kapellenstr. 25, 6200 Wiesbaden, Germany
- Age at test initiation: at least 2 months old
- Weight at test initiation: 303 - 565 mg (control group) and 314 - 600 mg (test group)
ACCLIMATION
- Acclimation period: no data
Study design
- Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- yes
- Total exposure duration:
- 14 d
- Post exposure observation period:
- no
Test conditions
- Test temperature:
- 20 ± 2 °C
- pH:
- 6.6 - 6.7
- Moisture:
- 40 % water content (based on dry weight)
- Details on test conditions:
- TEST SYSTEM
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
SOURCE AND PROPERTIES OF SUBSTRATE
- Composition: according to 88/302/EEC, C.8
OTHER TEST CONDITIONS
- Photoperiod: 24 hr
- Light intensity: approx. 400 - 600 lux
EFFECT PARAMETERS MEASURED: mortality after 7 and 14 days - Nominal and measured concentrations:
- Nominal: 1000 mg/kg dw
- Reference substance (positive control):
- yes
- Remarks:
- chloroacetamide
Results and discussion
Effect concentrations
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- - Mortality at end of exposure period: no mortality was observed
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: LC100 (14 d) = 100 mg/kg dw
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
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