Registration Dossier

Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

Administrative data

Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
20 Sep 1995 - 04 Oct 1995
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study conducted in compliance with GLP regulations

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report Date:
1995

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
EU Method C.8 (Toxicity for Earthworms: Artificial Soil Test)
Deviations:
yes
Remarks:
: average live weights of worms were not determined at test end
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Acrylic acid
- Substance type: pure substance
- Physical state: liquid
- Analytical purity: 99.78 % (mass, GC)
- Impurities (identity and concentrations): stabilised with 200 ppm hydrochinone monomethylether
- Stability under test conditions: stable
- ID No. of the testing laboratory: 0637/81718
- Sample No.: 1529/950420

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
yes
Details on preparation and application of test substrate:
- Chemical name of vehicle: water
- Concentration of vehicle in test medium (stock solution): 2.67 g/L

Test organisms

Test organisms (species):
Eisenia fetida
Animal group:
annelids
Details on test organisms:
TEST ORGANISM
- Common name: Earthworm
- Source: Eisenia GmbH & Co., Kapellenstr. 25, 6200 Wiesbaden
- Age at test initiation: at least 2 months old
- Weight at test initiation: 303-565 (control group) and 314-600 mg (test group)


ACCLIMATION
- Acclimation period: no data

Study design

Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
yes
Total exposure duration:
14 d
Post exposure observation period:
no

Test conditions

Test temperature:
20 +/- 2 °C
pH:
6.6 - 6.7
Moisture:
40 % water content (based on dry weight)
Details on test conditions:
TEST SYSTEM

- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4


SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Composition (if artificial substrate): according to 88/302/EEC, C.8


OTHER TEST CONDITIONS
- Photoperiod: 24 hr
- Light intensity: approx. 400-600 lux


EFFECT PARAMETERS MEASURED (with observation intervals): mortality after 7 and 14 days

Nominal and measured concentrations:
Nominal: 1000 mg/kg dw
Reference substance (positive control):
yes
Remarks:
chloroacetamide

Results and discussion

Effect concentrations
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
> 1 000 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
- Mortality at end of exposure period: no mortality
Results with reference substance (positive control):
- Results with reference substance valid? yes
- Relevant effect levels: LC100 (14 d) = 100 mg/kg dw

Applicant's summary and conclusion

Validity criteria fulfilled:
yes