Hydrogen [4-[4-(diethylamino)-2',4'-disulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene]diethylammonium, sodium salt

Endpoint summary

Additional information

Short-term toxicity to fish

Short term toxicity to fish was assessed for the target compound Hydrogen [4-[4-(diethylamino)-2', 4'-disulphonatobenzhydrylidene] cyclohexa-2, 5-dien-1-ylidene] diethylammonium, sodium salt (CAS no. 129-17-9) and its related read- across by reviewing a number of studies. The summary of the results are presented below:

In key study from UERL laboratory report; 2016 indicate that the Fish Acute Toxicity test conduct according to OECD Guideline 203 was conducted for Hydrogen [4-[4-(diethylamino)-2', 4'-disulphonatobenzhydrylidene] cyclohexa-2, 5-dien-1-ylidene] diethyl ammonium, sodium salt. The nominal concentration selected for the experiment were 6.25 mg/L, 12.5mg/L,25mg/L, 50mg/L, 100mg/L and Zebra Fish (Danio rerio)were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be > 100mg/L.

Whereas predicted study (from ECOSAR version 1.10 predicted model from EPI suite) for target chemical (Cas no. 129-17-9) indicate that the lethal concentrations i.e LC50 of test substance for fish was predicted to be 4480000 mg/l in freshwater and 5530000 mg/l in salt water for 96 hrs.

And other experimental study (The journal of Toxicological sciences,Vol.7.193-203;1982) for read across Acid Violet 6B (Cas no. 1694-09-3)indicate that the Fish toxicity study was conducted for 24 hrs and 48 hrs for evaluating the lethal concentration of test substance Acid Violet 6B on fish Oryzias latipes on the basis of mortality effect.

During experiment the Median Tolerance Limit i.e lethal concentration (LC50) for test substance Acid Violet 6B on fish Oryzias latipes was found to be 950 mg/l for 24 hrs. and 760 mg/l for 48 hrs.

From same experimental source for another read across substance Light green SF (Cas no. 5141-20-8) indicate that the lethal concentration i.e LC50 on fish Oryzias latipes was found to be 1000 mg/l for 24 hrs. and 48 hrs.

And for read across substance Guinea green B (Cas no. 4680-78-8) indicate that the lethal concentration i.e LC50 on fish Oryzias latipes was found to be 1000 mg/l for 24 hrs. and 990 mg/l for 48 hrs.

From authorative database ECOTOX the lethal concentration for read across N-Ethyl-N-[4[[4-[ethyl[(3-sulfophenyl)methyl]amino]phenyl](2-sulfophenyl)methylene]-2,5-cyclohexadien-1-ylidene]-3-sulfobenzenemethanaminium hydroxide inner salt disodium salt (Cas no. 3844-45-9)on fish was found to be Oncorhynchus gorbuscha (Pink Salmon) was found to be 398 mg/l for 96 hrs.

Based on above available studies for target chemical and its read across it is concluded that the substance Hydrogen [4-[4-(diethylamino)-2', 4'-disulphonatobenzhydrylidene] cyclohexa-2, 5-dien-1-ylidene] diethylammonium, sodium salt (CAS no. 129-17-9) not qualify for the aquatic classification as per the CLP criteria.

 

Short-term toxicity to aquatic invertebrates

Aim of this study was to assess the short term toxicity ofC.I. Food Blue 3,

to aquatic invertebrates daphnia magna. Study was performed according to the OECD guideline in a static system for the total exposure period of 48 hrs.

 

The stock solution 100 mg/L was prepared by dissolving yellow powder in reconstituted water. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. It was not possible to test at higher concentration because of limited solubility of tested sample (Changable sentence according to the chemical).Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.

 

The 8% inhibition for the test substance Food blue 3, in Daphnia magna was determined at 100 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the value, indicates that the substance is likely to be non-hazardous to aquatic invertebrates and cannot be be classified as per the CLP criteria.

 

Toxicity to aquatic algae and cyanobacteria

Aim of this study was to evaluate the nature of chemical test chemical when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201.The test solution 100 mg/l was prepared by dissolving yellow powder in OECD growth medium. Effects on the growth rate of the organism were studied (Changable). Various concentration were used.

With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs.

The median effective concentration (EC50) for the test substanceC.I. Food Blue 3, in algae was determined to be 56.3 mg/L on the basis of growth rate inhibition effects in a 72 hour study. Based on the EC50 value, indicates that the substance is likely to be hazardous/ non-hazardous to aquatic algae and cannot / can be be classified as aquatic chronic 3 category as per the CLP criteria.

 

Toxicity to microorganisms

Toxicity to micro organism was assessed for the target compound hydrogen [4-[4-(diethylamino)-2',4'-disulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene]diethylammonium, sodium salt (129-17-9) and its related read- across. The summary of the results are presented below:

The Toxicity to microorganism study predicted from SSS QSAR; 2016 for test substance hydrogen [4-[4-(diethylamino)-2',4'-disulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene]diethylammonium, sodium salt indicate that the effect concentration (EC10) of test substance was estimated to be 1115.2362 mg/l for Pseudomonas putida for 16 hrs.

Whereas experimental study (TOXICOLOGY AND APPLIED PHARMACOLOGY 39 , 111-117 (1977)) for read across substance Brilliant blue FCF (Cas no. 3844-45-9)indicate that the Brilliant blue FCF on the survival time of Paramaecium caudatum was measured microscopically for 20 min. Concentration of food dye in the bathing fluid was 0.1 or 1 .0%.

During the experiment the all the Paramaecium caudatum species survive even after the lapse of 14.68 min at dose concentration of 0.1%.

From same experimental source (i.e TOXICOLOGY AND APPLIED PHARMACOLOGY 39, 111-117 (1977)) for read across substance i.e Fast green FCF (Cas no. 2353-45-9) indicate that Fast green FCF on the survival time of Paramaecium caudatum was measured microscopically for 20 min. Concentration of food dye in the bathing fluid was 0.1 or 1 .0%.

During the experiment the 36.7% mortality observed of test organism i.e Paramecium caudatum after of 17.28 min.which is not consider to be very toxic.

Based on above available studies it is concluded that the substance hydrogen [4-[4-(diethylamino)-2',4'-disulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene]diethylammonium, sodium salt (129-17-9) was consider to be non toxic to micro organisms.