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EC number: 204-934-1 | CAS number: 129-17-9
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
28-days Manometric respirometry test followingthe OECD guideline 301Fto determine the ready biodegradability of the test itemHydrogen [4-[4-(diethylamino)-2', 4'-disulphonatobenzhydrylidene] cyclohexa-2, 5-dien-1-ylidene] diethylammonium, sodium salt (CAS No. 129-17-9)[UERL study report, Sustainability Support Services (Europe) AB (Report no. 129-17-9/02/2016/RBD), 2015]. The test system included control, test item and reference item. The concentration of test and reference item (Sodium Benzoate) chosen for both the study was 100mg/L, while that of inoculum was 10ml/l. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % Degradation was calculated using the values of BOD and ThOD for test item and reference item. The BOD28value of Hydrogen [4-[4-(diethylamino)-2', 4'-disulphonatobenzhydrylidene] cyclohexa-2, 5-dien-1-ylidene] diethylammonium, sodium salt (CAS No. 129-17-9) was observed to be 0.0335 mgO2/mg. ThOD was calculatedas 1.892 mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to manometric respirometry test was found to be 1.77%.Based on the results, the test item, under the test conditions, was found to be non-biodegradable at 20 ± 1°C over a period of 28 days.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
Various data for the test compound C.I. Food Blue 3(CAS No. 129-17-9) and the study for its read across substance were reviewed for the biodegradation end point which are summarized as below:
28-days Manometric respirometry test followingthe OECD guideline 301Fto determine the ready biodegradability of the test itemHydrogen [4-[4-(diethylamino)-2', 4'-disulphonatobenzhydrylidene] cyclohexa-2, 5-dien-1-ylidene] diethylammonium, sodium salt (CAS No. 129-17-9)[UERL study report, Sustainability Support Services (Europe) AB (Report no. 129-17-9/02/2016/RBD), 2015]. The test system included control, test item and reference item. The concentration of test and reference item (Sodium Benzoate) chosen for both the study was 100mg/L, while that of inoculum was 10ml/l. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % Degradation was calculated using the values of BOD and ThOD for test item and reference item. The BOD28value of Hydrogen [4-[4-(diethylamino)-2', 4'-disulphonatobenzhydrylidene] cyclohexa-2, 5-dien-1-ylidene] diethylammonium, sodium salt (CAS No. 129-17-9) was observed to be 0.0335 mgO2/mg. ThOD was calculatedas 1.892 mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to manometric respirometry test was found to be 1.77%.Based on the results, the test item, under the test conditions, was found to be non-biodegradable at 20 ± 1°C over a period of 28 days.
Estimation Programs Interface Suite (EPI suite, 2016) was run to predict the biodegradation potential of the test compound Hydrogen (4 -(4 -(diethylamino)-2',4'-disulphonatobenzhydrylidene)cyclohexa-2,5-dien-1-ylidene)diethylammonium, sodium salt(CAS no. 129 -17 -9). The screening testsready biodegradability of the test substance was calculated using thesoftware BIOWIN v4.10.The results indicate thatHydrogen (4 -(4 -(diethylamino)- -2',4 '-disulphonatobenzhydrylidene)cyclohexa-2,5 -dien-1-ylidene)diethylammonium, sodium saltis not expected to be readily biodegradable in nature.
Biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test substance CI acid violet 49 (J-CHECK, 2016). Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l. The percentage degradation of test substance was found to be 1% by BOD, 4% by TOC removal and 0% by HPLC in 28 days. Thus, the substance CI acid violet 49 is not expected to be readily biodegradable in water.
On the basis of above results for target and read across substance, it can be concluded that the test substance C.I. Food Blue 3 can be expected to be not readily biodegradable in nature.
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