Hydrogen [4-[4-(diethylamino)-2',4'-disulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene]diethylammonium, sodium salt

Endpoint summary

Additional information

Biodegradation

Biodegradation in water:

Various data for the test compoundC.I. Food Blue 3(CAS No. 129-17-9)and the study for its read across substance were reviewed for the biodegradation end point which are summarized as below:

28-days Manometric respirometry test followingthe OECD guideline 301Fto determine the ready biodegradability of the test itemHydrogen [4-[4-(diethylamino)-2', 4'-disulphonatobenzhydrylidene] cyclohexa-2, 5-dien-1-ylidene] diethylammonium, sodium salt (CAS No. 129-17-9)[UERL study report, Sustainability Support Services (Europe) AB (Report no. 129-17-9/02/2016/RBD), 2015]. The test system included control, test item and reference item. The concentration of test and reference item (Sodium Benzoate) chosen for both the study was 100mg/L, while that of inoculum was 10ml/l. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % Degradation was calculated using the values of BOD and ThOD for test item and reference item. The BOD₂₈value of Hydrogen [4-[4-(diethylamino)-2', 4'-disulphonatobenzhydrylidene] cyclohexa-2, 5-dien-1-ylidene] diethylammonium, sodium salt (CAS No. 129-17-9) was observed to be 0.0335 mgO₂/mg. ThOD was calculatedas 1.892 mgO₂/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to manometric respirometry test was found to be 1.77%.Based on the results, the test item, under the test conditions, was found to be non-biodegradable at 20 ± 1°C over a period of 28 days.

Estimation Programs Interface Suite (EPI suite, 2016) was run to predict the biodegradation potential of the test compound Hydrogen (4 -(4 -(diethylamino)-2',4'-disulphonatobenzhydrylidene)cyclohexa-2,5-dien-1-ylidene)diethylammonium, sodium salt(CAS no. 129 -17 -9). The screening testsready biodegradability of the test substance was calculated using thesoftware BIOWIN v4.10.The results indicate thatHydrogen (4 -(4 -(diethylamino)- -2',4 '-disulphonatobenzhydrylidene)cyclohexa-2,5 -dien-1-ylidene)diethylammonium, sodium saltis not expected to be readily biodegradable in nature.

Biodegradation study was conducted for 28 days for evaluating the percentage biodegradability of test substance CI acid violet 49 (J-CHECK, 2016). Concentration of inoculum i.e, sludge used was 30 mg/l and initial test substance conc. used in the study was 100 mg/l. The percentage degradation of test substance was found to be 1% by BOD, 4% by TOC removal and 0% by HPLC in 28 days. Thus, the substance CI acid violet 49 is not expected to be readily biodegradable in water.

On the basis of above results for target and read across substance, it can be concluded that the test substanceC.I. Food Blue 3can be expected to be not readily biodegradable in nature.

 

Biodegradation in water and sediment:

Estimation Programs Interface (EPI) Suite (2016) prediction model was run to predict the half-life in water and sediment for the test compound hydrogen [4-[4-(diethylamino)-2',4'-disulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene]diethylammonium, sodium salt(CAS No. 129 -17 -9).

Half-life period of hydrogen [4-[4-(diethylamino)-2',4'-disulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene]diethylammonium, sodium salt in water is estimated to be 60 days (1440 hrs.) while in sediment it is 541.6 days (13000 hrs). Based on these half-life values of hydrogen [4-[4-(diethylamino)-2',4'-disulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene]diethylammonium, sodium salt, it is concluded that the chemical is persistent in both water and in sediment.

 

Biodegradation in soil:

Based on EPI prediction Level III Fugacity Model (EPI suite, 2016), the estimated half-life period of Hydrogen(4-(4-(diethylamino)-2',4'-disulphonato

benzhydrylidene)cyclohexa-2,5-dien-1-ylidene)diethylammonium, sodium salt(CAS No. 129 -17 -9) in soil was obtained to be 120 days (1440 hrs).Based on this half-life value of Hydrogen (4-(4-(diethylamino)-2',4'-disulphonatobenzhydrylidene)cyclohexa-2,5-dien-1-ylidene)diethylammonium, sodium salt, it isconcluded that the chemical is not persistent in the soil environment.

On the basis of available information, the test substance C.I. Food Blue 3 can be considered to be not readily biodegradable in nature.

 

Bioaccumulation

Bioaccumulation: aquatic / sediment:

The estimated bioconcentration factor (BCF) for hydrogen [4-[4-(diethylamino)-2',4'-disulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene]diethylammonium, sodium salt is 3.162 L/kg, that does not exceed the bioconcentration threshold of 2000. Thus it is concluded that hydrogen [4-[4-(diethylamino)-2',4'-disulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene]diethylammonium, sodium salt is not expected to bioaccumulate in the food chain because it does not exceed the BCF criteria.

Transport and distribution:

Adsorption/desorption:

Experimental study report and studies of adsorption endpoint for the target chemical Hydrogen[4-[4-(diethylamino)-2',4'-disulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene]diethylammonium, sodium salt (Cas no. 129-17-9) and its read across chemical were reviewed to summarize as follows:  

The key study from experimental report (UERL laboratory report; 2016) for adsorption coefficient Koc in soil and in sewage sludge of target chemical hydrogen [4-[4 -(diethylamino)-2',4'-disulphonatobenzhydrylidene] cyclohexa-2,5-dien-1-ylidene]diethylammonium, sodium salt (CAS No. 129-17-9) was determined by the Reverse Phase High Performance Liquid Chromatographic method according to OECD Guideline No. 121 for testing of Chemicals. The reference substances were chosen according to estimated K_ocrange of the test substance and generalized calibration graph was prepared. The reference substances were chosen according to estimated K_ocrange of the test substance and generalized calibration graph was prepared. The reference substances were 4 chloroaniline, 4 methylaniline, N methylaniline, 2 Nitrophenol, Nitrobenzene, 4-Nitrobenzamide , N,N-dimethylbenzamide, N-methylbenzamide, Benzamide, phenanthrene having K_ocvalue ranging from 1.239 to 4.09. TheLog K_ocvalue was found tobe3.628 ± 0.135 at 25°C.

Other experimental study from HSDB authorative database for target indicate that the adsorption coefficient (Koc) of substance C.I. Acid Blue 1 was found to be 15 using a measured water solubility of 30,000 mg/land a regression-derived equation.

This Koc value suggests that C.I. Acid Blue 1 has very high mobility in soil.

And read across substance Brilliant blue (Cas no. 3844-45-9) from HSDB database indicate that the Brilliant Blue is expected to be mobile in soil; this compound will almost entirely exist in anion form in the environment and anions generally do not adsorb more strongly to soils containing organic carbon and clay than their neutral counterparts.

Whereas other supporting study for read across Fast Green FCF (Cas no. 2353-45-9) from same source i.e HSDB database indicate that the Fast Green FCF is expected to be mobile in soil; this compound will almost entirely exist in anion form in the environment and anions generally do not adsorb more strongly to soils containing organic carbon and clay than their neutral counterparts.

Thus based on above all experimental studies for target chemical and for read across substances it is concluded that the target chemical Hydrogen[4-[4-(diethylamino)-2',4'-disulphonatobenzhydrylidene]cyclohexa-2,5-dien-1-ylidene]diethylammonium, sodium salt (Cas no. 129-17-9) have highly to moderate mobility in soil/sediment.