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EC number: 248-363-6 | CAS number: 27247-96-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Report Date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. certificate)
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Type:
- impurity
Test organisms
- Test organisms (species):
- Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- Strain No.: CCAP 278/4.
Reason for this choice: species commonly used in Europe for aquatic toxicity testing and recommended in OECD and EEC guidelines.
Origin: Culture Collection of Algae and Protozoa (CCAP), SAMS Research Services Ltd, Dunstaffnage Marine Laboratory, Dunbeg, OBAN, Argyll PA37 1QA, Scotland.
Cultured at: CIT.
Culture method: the algae are cultured under sterile conditions and maintained at exponential growth rate.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- Any requirement but water hardness will be measured once in test water before the start of the test.
- Test temperature:
- Should be set between 21°C and 24°C and not varying by more than ± 2°C throughout the test.
- pH:
- between 7.0 and 7.2 at the beginning of the test and should not deviated by more than 1.5 units in the control throughout the test
- Nominal and measured concentrations:
- 0.01, 0.1, 1 and 6 mg/L
- Details on test conditions:
- Test vessels were sealed and headspace was kept at minimum with full conical flasks. In addition, an addition of 0.3 g/L of NaHCO3 then a pH adjustment to pH 7.1 +/-0.1 of OECD medium was performed (to prevent limitation of growth coming from CO2 depletion and important increasing pH).
A preliminary test, including a combined range-finding and limit test, preceded the definitive test. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 5.35 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.26 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 3.22 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 1.54 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.57 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.76 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- cell number
- Details on results:
- The results showed a clear dose-response relationship which is why the ErC(x)s and EyC(x)s values were calculated from the nominal concentrations by statistical analysis. (The 24, 48 and 72-hours ErC(x)s and 72-hours EyC(x)s calculations are reported in Appendix 5 for information).
However all measured concentrations without were below the limit of quantification (0.8 mg/L) at the test concentration of 3.75 mg/L or less.
Therefore according to these analytical results it was assumed that the nominal concentrations were higher and not representative of the concentrations actually tested.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In a 72-hour algal growth inhibition test with Pseudokirchneriella subcapitata, the EC50 of 2 Ethylhexyl nitrate was < 0.8 mg/L (nominal) based on average specific growth rate (ErC50) and < 0.8 mg/L based on yield (EyC50).
However, there is sufficient uncertainty in what was the actual concentration that caused the algal growth inhibition that we should not use the value of <0.8 mg/l as the EC50 value for 2-ethylhexyl nitrate.
We therefore propose that you use the nominal concentration to calculate the EC50:
72 hour EC50 (Growth Rate) = 3.22 mg/l
72 hour EC50 (Yield) = 1.57 mg/l
The use of nominal concentrations can be justified on the basis of the difficulties incurred in measuring dissolved concentrations.
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