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EC number: 204-001-9 | CAS number: 112-73-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012-05-02 to 2012-05-05
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with GLP and no defiencies
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- Bis(2-butoxyethyl) ether
- EC Number:
- 204-001-9
- EC Name:
- Bis(2-butoxyethyl) ether
- Cas Number:
- 112-73-2
- Molecular formula:
- C12H26O3
- IUPAC Name:
- 1-[2-(2-butoxyethoxy)ethoxy]butane
- Details on test material:
- - Name of test material (as cited in study report): Diethyleneglycoldibutylether
- Molecular formula: C12H26O3
- Molecular weight: 218.34 g/mol
- Physical state: Liquid, colourless
- Stability under test conditions: Stable
- Storage condition of test material: Room temperature, protected from light, in original container
- Water solubility: 2.8 g/L (20 °C, DIN 51757)
- Density: 0.883 g/cm3 (1013 hPa)
- Log Pow: 1.92
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 6.25 – 12.5 – 25.0 – 50.0 – 100 mg/L.
- Sampling method: The test item Diethyleneglycoldibutylether was analytically verified by GC-MS in the fresh media (0 h) and old media (48 h) of all concentration levels and the control.
Sampling from the freshly prepared media was carried out immediately after homogenisation. Samples of the old media were taken from the approximate centre of the glass flasks. Recoveries of the test item should be within ± 20% of the nominal concentrations.
- Sample storage conditions before analysis:All original samples were stored at 6 ± 2 °C until sample preparation. Prepared samples were stored at room temperature until analysis.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A stock solution of 100 mg/L was freshly prepared with dilution water. Dispersion treatment was agitation.
- Eluate: Dilution water
- Differential loading: 6.25 – 12.5 – 25.0 – 50.0 – 100 mg/L
- Controls: Dilution water without test item under the same conditions as the groups.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna (STRAUS)
- Strain: Clone 5
- Source: Institut für Wasser-, Boden- Lufthygiene (WaBoLu), further own breeding
- Age at study initiation (mean and range, SD): 2 to 24 h
- Method of breeding: In 2-3 L glass vessels with appr. 1.8 L culture medium, at 20 +/- 2 °C, in an incubator, 16 h illumination, illumination strength max. 20 µExm-2 x s-1
- Feeding during test: No feeding
ACCLIMATION
- Acclimation period: 2 h
- Acclimation conditions (same as test or not): Same as test
- Type and amount of food: No feeding
- Health during acclimation (any mortality observed): Healthy, no mortality observed
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- No
Test conditions
- Hardness:
- Dilution water at test start: 249 (mg CaCO3/L)
- Test temperature:
- 21.8 °C
- pH:
Water Quality Parameters at 0 h (Fresh Media)
(measured in one additional replicate per concentration level and control)
Diethyleneglycoldibutylether
Nominal
Test Item Concentration
[mg/L] pH-Value
100 7.88
50.0 7.87
25.0 7.88
12.5 7.88
6.25 7.88
Control 7.91
Water Quality Parameters after 48 h (Old Media)
(measured in all replicates)
Diethyleneglycoldibutylether
Nominal
Test Item Concentration
[mg/L] pH-Values
Replicates
1 2 3 4
100 7.55 7.59 7.59 7.54
50.0 7.64 7.62 7.62 7.62
25.0 7.69 7.64 7.64 7.66
12.5 7.70 7.69 7.69 7.68
6.25 7.56 7.57 7.71 7.70
Control 7.55 7.55 7.45 7.57- Dissolved oxygen:
- Water Quality Parameters at 0 h (Fresh Media)
(measured in one additional replicate per concentration level and control)
Diethyleneglycoldibutylether
Nominal
Test Item Concentration
[mg/L] Dissolved O2-Concentration
[mg/L]
100 9.59
50.0 9.45
25.0 9.57
12.5 9.31
6.25 9.57
Control 8.34
Water Quality Parameters after 48 h (Old Media)
(measured in all replicates)
Diethyleneglycoldibutylether
Nominal
Test Item Concentration
[mg/L] Dissolved O2 -Concentration [mg/L]
Replicates
1 2 3 4
100 8.29 7.98 7.77 7.06
50.0 8.09 7.89 7.56 7.54
25.0 8.14 7.77 7.76 7.63
12.5 8.37 8.16 8.54 8.44
6.25 8.27 8.28 8.40 8.36
Control 7.70 7.78 7.63 6.79 - Salinity:
- Not measured, freshwater
- Nominal and measured concentrations:
- Please refer to "Any other information on materials and methods"
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers, 50 mL
- Type (delete if not applicable): closed with parafilm
- Material, size, headspace, fill volume: Glass beakers (4 cm ID x 7 cm H), 50 mL
- Aeration: No
- Renewal rate of test solution (frequency/flow rate): One application at test start, static test
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): None
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Acc. to Directive 92/69/EEC Method C.2, Annex 1
- Conductivity: Dilution water at test start: 645 µS/cm
- Culture medium different from test medium: Elendt M4 acc. to ELENDT (1990), modified to a total hardness of 160 to 180 mg CaCO3/L
- Intervals of water quality measurement: At test start and test end
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16:8 light:dark cycle
- Light intensity: Diffuse light, illumination strength max. 20 µE x m-2 x s-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Immobility was determined in all groups after 24 h and 48 h. An animal was considered to be immobile, if it was not able to swim within 15 sec after gentle agitation of the test vessel.
TEST CONCENTRATIONS
- Range finding study:
Immobilization Rates in the non GLP Preliminary Range Finding Test
(n = 20, divided into 2 replicates with 10 daphnids each)
Nominal
Test Item Concentration
[mg/L] IMMOBILIZATION [%]
24 h 48 h
Replicates Replicates
1 2 MV 1 2 MV
100 70 60 65 90 100 95
10 0 0 0 0 0 0
1 0 0 0 0 0 0
Control 0 0 0 0 0 0 - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 42.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CI 34.5-47.0 mg/L
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- 28.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CI 22.0-37.9 mg/L
- Details on results:
- - Mortality of control: 0 %
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: No - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EC50/LC50: 1.76 (CI 1.50 - 2.01) mg/L - Reported statistics and error estimates:
EC100-values after 24 and 48 h, the NOEC and LOEC after 48 h were deduced directly from the observation data, without mathematical calculation.
EC10- and the EC50-values were calculated by sigmoidal dose-response regression. The confidence intervals were calculated from the best-fit values, the standard error and the t-distribution with the software GraphPad Prism5, wherever possible. The concentration effect relationships are shown graphically.
Any other information on results incl. tables
Immobilization Rates in the Definitive Test after 24 and 48 h of Exposure
(n = 20, divided into 4 replicates with 5 daphnids each)
Diethyleneglycoldibutylether |
IMMOBILIZATION [%] |
|||||||||
Nominal Test Item Concentration [mg/L] |
24 h |
48 h |
||||||||
Replicates |
Replicates |
|||||||||
1. |
2. |
3. |
4. |
MV |
1. |
2. |
3. |
4. |
MV |
|
100 |
40 |
80 |
40 |
60 |
55 |
100 |
100 |
100 |
80 |
95 |
50.0 |
60 |
40 |
20 |
40 |
40 |
80 |
80 |
80 |
40 |
70 |
25.0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
20 |
5 |
12.5 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
6.25 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the nominal concentrations of the test item Diethyleneglycoldibutylether the 48-h EC50 for Daphnia magna was estimated to be 42.5 mg/L (95 % confidence limits: 34.5 – 47.0 mg/L). The NOEC after 48 h was 25.0 mg/L. The LOEC after 48 h was 50.0 mg/L.
- Executive summary:
In the acute immobilization test with Daphnia magna (STRAUS), the effects of the test item Diethyleneglycoldibutylether(batch number: DEG4136740) were determined according to OECD 202 (2004) from 2012-05-02 to 2012-05-05, with the definitive exposure phase from 2012-05-02 to 2012-05-04, at Dr.U.Noack-Laboratorien, Käthe-Paulus-Str.1, 31157 Sarstedt, Germany.
The study was conducted in sealed glass flasks under static conditions over a period of 48 h with 5concentration levels of the test item Diethyleneglycoldibutylether in the range of 6.25 to 100 mg/L prepared with dilution water in a geometric series with a dilution factor of 2. Twenty daphnids were exposed to each concentration level and the control.
The concentrations of the test item Diethyleneglycoldibutyletherwere analytically verified by GC-MS in the fresh media (0 h) and in the old media (48 h) of all concentration levels and the control.
The measured concentrations of the test item Diethyleneglycoldibutyletherin the fresh media (0 h) were in the range of 95 to 111 % of the nominal values. The measured concentrations of the test item in the old media (48 h) were in the range of 93 to 120 % of the nominal values, indicating that the concentrations of the test item Diethyleneglycoldibutylether were successfully maintained for the duration of the test.
The endpoints were assessed based on the nominal concentrations of the test item Diethyleneglycoldibutylether(Table 1).
The water quality parameters (pH-value and dissolved oxygen concentration), measured at 0 and 48 h, were determined to be within the acceptable limits. The validity criteria of the test guideline were fulfilled.
EC10-, EC50- (with Confidence Interval), EC100 - Values, NOEC and LOEC
(based on the nominal concentrations of the test item Diethyleneglycoldibutylether)
Endpoints
(with 95 % confidence limits)
Test
Duration
[h]
Diethyleneglycoldibutylether
Nominal Concentration
of the Test Item
[mg/L]
EC10
24
33.6 (CI: 28.0 – 41.2)
48
28.5 (CI: 22.0 – 37.9)
EC50
24
61.9 (CI: 49.0 – > 100)
48
42.5 (CI: 34.5 – 47.0)
EC100
24
> 100
48
> 100
NOEC
48
25.0
LOEC
48
50.0
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