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EC number: 202-895-5
CAS number: 100-85-6
Table 1: Comparison of Biodegradation of the Test Item in Bottles
A and B– Experiment 2
Mean A and B
1): Absolute difference in biodegradation between bottles A and B
Table 2: Comparison of Biodegradation of the Test Item in Bottles
A and B– Experiment 3
In a GLP-compliant Manometric Respirometry Test according to OECD
guideline 301 F, the substance was assessed for its ready
biodegradability. The test was performed three times: the first
experiment was invalid and because the second experiment showed >100%
biodegradation, a third experiment was performed to confirm the findings
of the second experiment.
The substance was tested in duplicate at a target concentration of 28
and 36 mg/L, corresponding to a ThOD of 64 and 81 mg O2/L, in Experiment
2 and 3 respectively. The exposure period was 28 days and two inoculum
blanks, a procedure control and a toxicity control were included. The
substance was added directly into the test bottles. The test solutions
were continuously stirred during the test to ensure optimal contact
between the substance and test organisms.
In Experiment 2, 130% and 116% biodegradation was observed in two
replicate bottles. In Experiment 3, 95% biodegradation was observed in
both replicate bottles. Average biodegradation of at least 60% was
reached within a 10 -day window in both experiments. The toxicity
control reached 85% biodegradation within 14 days in Experiment 2, and
less than 25% in Experiment 3. The results of the toxicity control in
the second experiment are in line with what can be expected based on the
biodegradation profile of the test item. Therefore, the test item was
assumed not to inhibit microbial activity.
The criterion for ready biodegradability (at least 60% biodegradation
within a 10 -day window) was met. Based on the outcome of the two
experiments, the substance is considered to be readily biodegradable in
the Manometric Respirometry Test. All validity criteria were met, thus
the study was considered to be valid and reliable without restriction.
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