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EC number: 202-895-5
CAS number: 100-85-6
Table 1: Final Test – Overview of Results
Mean respiration rate
% Inhibition of the respiration rate (mean value)
(mg O2/L h)
(mg O2/g h) (a)
(a) The amount of suspended solids in the final test
mixture was 1.5 g/L.
* Statistically significantly different compared to control.
In a 3 -h activated sludge respiration inhibition study conducted
according to OECD Guideline 209 and according to GLP Principles,
microorganisms in activated sludge were exposed to the test item at
nominal concentrations of 10, 32, 100, 320 and 1000 mg/L. The test item
concentration was corrected for water content, reported concentrations
are based on the pure test item.
The test item was not toxic at or below a concentration of 32 mg/L
(NOEC), and inhibited respiration up to 87% in the highest test
concentration. The EC10 was 200 mg/L (95% confidence interval: 170 - 235
mg/L), the EC50 was 481 mg/L (95% confidence interval: 440 - 526 mg/L).
The study met all validity criteria and is considered reliable without
The test item was not toxic to waste water bacteria (activated sludge)
at or below a concentration of 32 mg/L (NOEC, based on the pure test
item, corrected for water content). The EC10 was 200 mg/L (95%
confidence interval: 170 - 235 mg/L). The EC50 was 481 mg/L (95%
confidence interval: 440 - 526 mg/L).
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