Registration Dossier

Administrative data

Endpoint:
toxicity to terrestrial arthropods: short-term
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Only secondary literature. Original reference is not available.

Data source

Referenceopen allclose all

Reference Type:
secondary source
Title:
1,2-Dichlornitrobenzole (1,2-Dichlor-3-nitrobenzol, 1,2-Dichlor-4-nitrobenzol)
Author:
BUA (Beratergremium umweltrelevanter Altstoffe) (BUA)
Year:
1990
Bibliographic source:
Gesellschaft Deutscher Chemiker (Hrsg Behret) Bd 52, VCH (Verlag Chemie), Weinheim
Reference Type:
publication
Title:
Chemical Mutagenesis testing in Drosophila. IV. Results of 45 Coded Compounds Tested for the National Toxicology Program
Author:
Yoon J.S., Mason J.M., Valencia R., Woodruff R.C., Zimmering S.
Year:
1985
Bibliographic source:
Environ. Mutagen. 7, 349-367

Materials and methods

Principles of method if other than guideline:
acute toxicity of 2,3-dichloronitrobenzene to Drosophila melanogaster was tested by injection of a solution the test substance into the fly and by feeding of a solution of the test substance
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
not specified

Test substrate

Vehicle:
yes
Details on preparation and application of test substrate:
- a stock solution was prepared in 0.6-3% of ethanol in water
- in case of the injection test the stock solution was diluted with a 0.7% solution of sodium chloride in water
- in case of the feeding test the stock solution was diluted with a 5% solution of saccharose in water

Test organisms

Test organisms (species):
Drosophila melanogaster
Animal group:
Diptera (fruit fly (small))
Details on test organisms:
- only male flies were tested

Test conditions

Nominal and measured concentrations:
- in both experiments (injection + feeding) a nominal concentration of 20 mg/l was tested

Results and discussion

Any other information on results incl. tables

Injection test (24 hours)

LC11 = 20 mg/l

Sterility rate: 1%

 

Feeding test (72 hours)

LC30 = 20 mg/l

Sterility rate: 2%

Applicant's summary and conclusion