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Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Administrative data

Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: acceptable, well documented publication which meets basic scientific principles

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Quantitative structure-activity relationships for the toxicity and bioconcentration factor of nitrobenzene derivatives towards the guppy (Poecilia reticulata).
Author:
Deneer JW, Sinnige TL, Seinen W, Hermens JLM
Year:
1987
Bibliographic source:
Aquat. Toxicol. 10: pp 115-129.
Reference Type:
secondary source
Title:
1,2-Dichloronitrobenezole, BUA-Stoffbericht 52 (Juli 1990) [Report in German]
Author:
GDCh, BUA
Year:
1990
Bibliographic source:
publisher: VCH Verlagsgesellschaft, Weinheim; ISSN: 0179-2601

Materials and methods

Principles of method if other than guideline:
The bioconcentration factor of a series of nitrobenzenes derivatives was determined for the guppy.
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 2,3-dichloronitrobenzne
- Analytical purity: >98 %

Sampling and analysis

Details on sampling:
- Sampling intervals/frequency for test organisms: After 1 and 2 days of exposure two guppies were taken for analysis; at the end of day three all remaining fish were taken.

Test organisms

Test organisms (species):
Poecilia reticulata
Details on test organisms:
TEST ORGANISM
- Common name: guppy
- female guppies were used
- Age at study initiation: 5 to 8 month old
- Weight at study initiation: wet weight varied from 60 to 450 mg at the end of the experiment; mean fat content was 8 +/-2 %
- Food type: commercial fishfood
- Frequency: daily


ACCLIMATION
- Acclimation period: at least 12 days

Study design

Route of exposure:
aqueous
Test type:
semi-static
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
3 d

Test conditions

Hardness:
25 mg/l CaCO3
Details on test conditions:
TEST MEDIUM / WATER PARAMETERS
- water for all experiments: standard water (SW) prepared according to Alabaster and Abram (1964), with corresponds to very soft tap water (hardness: 25 mg/l as CaCO3)

TEST SYSTEM
- Test vessel: glas jars of 1.5 l containing 1 l of solution
- Renewal rate of test solution: daily
- No. of organisms per vessel: 9 female guppies

TEST MEDIUM / WATER PARAMETERS
- water for all experiments: standard water (SW) prepared according to Alabaster and Abram (1964), with corresponds to very soft tap water (hardness: 25 mg/l as CaCO3)

Results and discussion

Bioaccumulation factoropen allclose all
Type:
BCF
Value:
1 027
Basis:
whole body w.w.
Remarks on result:
other: Conc.in environment / dose:840 µg/l
Type:
BCF
Value:
82
Basis:
total lipid content
Remarks on result:
other: average lipid content: 8 +/- 2 %
Remarks:
Conc.in environment / dose:840 mg/l

Any other information on results incl. tables

results:

BCF: 1027 +/-95 (N=7); whole body w.w.

log BCF: 3.01 +/- 0.04; whole body w.w.

BCF: 82 +/- 20; total lipid content

In same preliminary experiments it was established that the uptake of the chemicals proceeded very fast, thus enabling the use of this short-term assay.

Applicant's summary and conclusion