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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions; basic data given

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1993

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
Deviations:
no
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 2,3-dichloronitrobenzene
- Analytical purity: no data

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
mixture of sewage, soil and natural water
Details on inoculum:
Concentration of activated sludge: 30 ppm
Duration of test (contact time):
4 wk
Initial test substance concentration
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimationopen allclose all
Parameter followed for biodegradation estimation:
O2 consumption
Parameter followed for biodegradation estimation:
test mat. analysis
Details on study design:
Test Equipment: Improved type for a volatile substance
Reference substance
Reference substance:
not specified

Results and discussion

% Degradationopen allclose all
Parameter:
% degradation (O2 consumption)
Value:
4
Sampling time:
4 wk
Remarks on result:
other: BOD
Parameter:
% degradation (test mat. analysis)
Value:
5
Sampling time:
4 wk
Remarks on result:
other: HPLC

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Interpretation of results:
under test conditions no biodegradation observed
Executive summary:

MITI (1993)

The ready biodegradability was studied with a method corresponding to the OECD TG 301C, Modified MITI (I) test. The test concentration of 1,2-dichloro-3-nitrobenzene was 100 mg/l and activated sludge concentration 30 mg/l. In the aerobic study, the degradation measured as Biochemical Oxygen Demand (BOD) was 4 % after 4 weeks. The direct HPLC analysis obtain 5% degradation of 1,2-dichloro-3-nitrobenzene.

1,2-dichloro-3-nitrobenzene is not readily biodegradable under the test conditionen.