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Ecotoxicological information

Short-term toxicity to fish

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Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1990

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
yes
Remarks:
1.) photoperiod 14 to 10 hours instead of 12 to 16 hours, 2.) no daily measurement of pH and DOC, 3.) origin and purity of test substance not reported
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Dichlornitrobenzole (2,3-Isomer) and 2,3-Dichlornitrobenzol
- Analytical purity: not given
=> no informations on test substance are given

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes
Details on test solutions:
- number of test concentrations: 5
- Controls: one control without test substance and one control containing the highest concentration of the vehicle acteone used (158 mg/l)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Acetone

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name: zebrafish
- Strain: Hamilton-Buchanan
- Source: Doller Zierfischzüchterei, 5950 Finnentrop-Serkenrode, Germany
- Length at study initiation (length definition, mean, range and SD): 2.0 +/- 1.0 cm
- Feeding during test: none

ACCLIMATION
- Acclimation period: at least 12 days before starting the test
- Type and amount of food: daily feeding with TetraMin

=> only healthy fish were tested

Study design

Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Test temperature:
23 +/- 1 °C
pH:
8.15 +/- 0.2
Dissolved oxygen:
oxygen saturation in the basin connected upstream to the test basin => test basins were not ventilated due to the volatile character of the test substance
Nominal and measured concentrations:
basin 1 - n: 12.5 mg/l; a: 13.4 mg/l
basin 2 - n: 6.25 mg/l; a: 6.28 mg/l
basin 3 - n: 3.13 mg/l; a: 2.82 mg/l
basin 4 - n: 1.57 mg/l; a: 1.59 mg/l
basin 5 - n: 0.78 mg/l; a: 0.74 mg/l
Details on test conditions:
TEST SYSTEM
- Type: closed
- Aeration: oxygen saturation in the basin connected upstream to the test basin => test basins were not ventilated due to the volatile character of the test substance
- No. of organisms per vessel: 10

OTHER TEST CONDITIONS
- Photoperiod: 14 -10 hours
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
2.6 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
3.8 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
6.28 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
3.1 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
4.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
6.25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.

Any other information on results incl. tables

recovery rate of 2,3 -dichloronitrobenzene: 80.2 % (± 5.7)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes